2489 UV/Vis Detector

2489 UV/Visible (UV/Vis) Detector

The most sensitive and versatile dual-wavelength absorbance detector available

The most sensitive and versatile dual-wavelength absorbance detector available

Whether yours is a laboratory performing routine UV-based applications or pushing the limits of detection with low-level impurity analyses, the Waters 2489 UV/Visible (UV/Vis) Detector is the best detection choice for performance, reliability, and usability. 

The 2489 UV/Vis Detector is the most sensitive and versatile dual-wavelength absorbance detector available for HPLC and UHPLC. It is designed to combine a high energy deuterium source, a precise optical design, and low noise, high speed electronics all in one detector. These advanced capabilities take UV/Vis detection performance to an unprecedented new level.

2489 UV/Visible (UV/Vis) Detector. 2489 UV/Visible (UV/Vis) Detector

Specifications

Wavelength range

190 to 700 nm

Bandwidth

≤5 nm

Wavelength accuracy

±1 nm (via patented Erbium filter)

Wavelength repeatability

±0.1 nm

Linearity

≤5% at 2.5 AU, propylparaben, 257 nm, analytical flow cell

Baseline noise, single wavelength

≤5 x 10-6 AU peak to peak, 230 nm, 10 points/s, 1.0 s, 30 s segments, dry analytical flow cell

Baseline noise, dual wavelength

≤35 x 10-6 AU peak to peak, 230 nm, 280 nm, 1 point/s, 2.0 s, 30 s segments, dry analytical flow cell

Drift

≤1 x 10-4 AU/hour, 239 nm, 2 points/s, 1.0 s, 30 s segments, dry analytical flow cell

Measurement range

0.0001 to 4.0000 AU

Sampling rate

Up to 80 points/s

Light source

Deuterium arc lamp

Warranty: 2000 hours or 1 year (whichever comes first)

Flow cell design

Patented TaperSlit

Path length

10 mm (analytical cell)

Cell volume

16.3 μL (analytical cell)

Pressure limit

1000 psi (analytical cell)

Wetted materials

Alliance HPLC/ACQUITY Arc: 316 stainless-steel, fused sililca, PEEK, fluoropolymer ACQUITY Arc Bio: fluoropolymer, fused silica, MP35N, PEEK, titanium

Overview

  • Identify and quantitate major and minor components in the same run with a linear range of 2.5 AU
  • Monitor absorbance at two user-selectable wavelengths, resulting in more analyte information per run, and increased productivity
  • Achieve confidence for optimal system usage with lamp serial numbers, hours, and number ignition tracking provides

Recommended Use: The best dual-wavelength absorbance detection choice for performance, reliability, and usability.

Features Header

Features Header

Filled with innovation - designed for usability

The 2489 UV/Vis Detector is the most sensitive and versatile dual-wavelength absorbance detector available for HPLC. The detector’s advanced features take UV/Vis detection performance to a new level, with: 

  • Enhanced sensitivity via patented TaperSlit flow cell, which channels light through the cell for better energy throughput while minimizing RI effects
  • Low noise performance (<5 µAU)
  • Lamp optimization for the best s/n performance over the lifetime of a Deuterium lamp
  • Flexible sampling rates, from 1 to 80 Hz, for normal, and fast LC separations
  • Independent optimization of sampling rates and filter time constants gives you the ability to tune your detector to your separation goals
  • Enhanced thermal wander management reduces baseline wander due to fluctuations caused by ambient conditions
  • Flow cell options to match your separation scale and type
  • Accessible detector components for easy operation and maintenance

Setting the standard for trace impurity analysis

The 2489 UV/Vis Detector offers simplicity for routine UV-based applications and high sensitivity for low-level impurity analysis. The detector’s extended linear range of 2.5 AU enables the identification and quantitation of both major and minor components in the same run - a key benefit for trace impurity or metabolism studies.

More analyte information per run

Dual wavelength capability is standard on the 2489 UV/Vis Detector. This gives you the flexibility to simultaneously monitor absorbance at two user-selectable wavelengths, resulting in more analyte information per run, and increased productivity. The two wavelength channels can be overlaid, ratioed, and/or summarized within the data system for better qualitative data.

Optimal performance across your workflows

Whether you work in research and development, analytical development, or QA/QC, the 2489 UV/Vis Detector is designed to meet your needs with a wide range of flow cell options and integrated features, while providing optimal flexibility, usability, and performance for both routine and complex samples.

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2489 UV/Vis Detector

The Waters 2489 UV/Visible (UV/Vis) Detector is renowned as the most sensitive and versatile dual-wavelength absorbance detector available for high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) systems. With its advanced design features, this detector is perfectly suited for laboratories that require both high sensitivity for trace-level analysis and robust reliability for routine applications.

Combining a high-energy deuterium source with precise optical engineering and low-noise, high-speed electronics, the 2489 UV/Vis Detector brings an unprecedented level of performance to UV/Vis detection. This exceptional sensitivity is critical for detecting low-level impurities and ensuring the accuracy of quantitative analyses, making the detector a valuable tool for a variety of scientific disciplines, including pharmaceuticals, environmental testing, and food safety.

A standout feature of the 2489 UV/Vis Detector is its dual-wavelength capability, which allows users to monitor absorbance at two user-selectable wavelengths simultaneously. This functionality not only increases the amount of analyte information obtained per run but also significantly boosts laboratory productivity by enabling more comprehensive analysis in a single pass. The ability to overlay, ratio, and summarize two wavelength channels within the data system provides enhanced qualitative data interpretation, which is vital for complex mixture analyses and method development.

The detector's extended linear range of up to 2.5 absorbance units (AU) facilitates the simultaneous identification and quantitation of major and minor components, which is particularly beneficial for applications involving trace impurity or metabolism studies. This broad range ensures that the detector can handle highly absorbing samples without compromising linearity or accuracy.

Further enhancing its usability, the 2489 UV/Vis Detector incorporates several innovative design elements. The patented TaperSlit flow cell design optimizes the path of light through the cell, maximizing energy throughput while minimizing refractive index effects and dispersion. This results in superior peak shapes and ultra-low noise performance (less than 5 µAU), essential for detecting subtle changes in sample composition.

To maintain optimal performance throughout the detector's lifespan, the system features lamp optimization technology that ensures the best signal-to-noise performance. This is complemented by advanced thermal wander management, which reduces baseline wander caused by ambient temperature fluctuations, thus maintaining stability and reproducibility in analytical results.

The versatility of the 2489 UV/Vis Detector is further evidenced by its wide range of flow cell options, designed to match different separation scales and types. These options, coupled with flexible sampling rates from 1 to 80 Hz, allow for fine-tuning of the detector to meet specific analytical requirements, whether for fast LC separations or more traditional approaches.

The Waters 2489 UV/Vis Detector sets a new standard for dual-wavelength absorbance detection. Its combination of sensitivity, versatility, and user-friendly design makes it an essential asset for laboratories aiming to enhance their analytical capabilities. Whether for routine analysis or cutting-edge research, this detector provides the performance, reliability, and usability needed to meet the most demanding scientific challenges.