This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of sulfa drugs.
The compounds used in this study are -
Compounds |
USP Tailing Factors |
---|---|
Sulfanilamide |
1.27 |
Sulfadiazine |
1.19 |
Sulfathiazole |
1.18 |
Sulfamerazine |
1.15 |
Sulfamethazine |
1.12 |
Succinylsulfathiazole |
1.11 |
Column: |
Symmetry C8, 3.9 x 150 mm, 5 μm |
Guard column: |
Sentry Guard Column 3.9 x 20 mm, 5 μm |
Part numbers: |
Column - WAT046970, Guard - WAT054250 |
Mobile phase: |
Water/methanol/glacial acetic acid 79:20:1 |
Flow rate: |
1.0 mL/min |
Injection volume: |
10 μL of sample with components at 10 to 39 μg/mL |
Detection: |
UV @ 254 nm |
WA31763.154, June 2003