• Application Note

DNPH Derivatives - pH 2.45

DNPH Derivatives - pH 2.45
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of DNPH derivatives.

Introduction

The compounds used in this study are –

  1. Formaldehyde-DNPH
  2. Acetaldehyde-DNPH
  3. Acetone-DNPH
  4. Crotonaldehyde-DNPH
  5. Cyclohexanone-DNPH

Experimental

Conditions

Column:

Xterra Phenyl, 4.6 x 150 mm, 5 μm

Part number:

186001146

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

50 mM HCOOH, pH 2.45

Flow rate:

1.4 mL/min

Injection volume:

10 μL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient

Results and Discussion

Featured Products

WA20738.043, June 2002

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