This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of CNS depressants.
Compounds studied in this application brief are shown below.
Column: |
SunFire C18 4.6 x 150 mm, 5.0 μm (p/n: 186002559) |
Mobile phase A: |
Water |
Mobile phase B: |
Acetonitrile |
Mobile phase C: |
2% CH3COOH in water |
Flow rate: |
1 mL/min |
Injection volume: |
10 μL |
Isocratic Mobile Phase Conditions: |
55% A, 35% B, 10% C |
Sample concentration: |
10 μg/mL in water |
Temperature: |
30 °C |
Detection: |
UV @ 254 nm |
Instrument: |
Alliance 2695 with 2996 PDA |
Column: |
SunFire C8 4.6 x 150 mm, 5.0 μm (p/n: 186002737) |
Mobile phase A: |
Water |
Mobile phase B: |
Acetonitrile |
Mobile phase C: |
2% Acetic acid in water |
Flow rate: |
1 mL/min |
Injection volume: |
10 μL |
Isocratic Mobile Phase Conditions: |
55% A, 35% B, 10% C |
Sample concentration: |
10 μg/mL in water |
Temperature: |
30 °C |
Detection: |
UV @ 254 nm |
Instrument: |
Alliance 2695 with 2996 PDA |
Column: |
SunFire C8 4.6 x 100 mm, 5.0 μm (p/n: 186002731) |
Mobile phase A: |
Water |
Mobile phase B: |
Acetonitrile |
Mobile phase C: |
2% Acetic acid in water |
Flow rate: |
1 mL/min |
Injection volume: |
10 μL |
Isocratic Mobile Phase Conditions: |
55% A, 35% B, 10% C |
Sample concentration: |
10 μg/mL in water |
Temperature: |
30 °C |
Detection: |
UV @ 254 nm |
Instrument: |
Alliance 2695 with 2996 PDA |
WA41889, March 2005