Lipid nanoparticles (LNPs) are used to encapsulate and deliver nucleic acid, protein, and small molecule drugs into targeted cells. Celebrated as the delivery vehicles for the first mRNA vaccines, LNPs continue to be the focus of intense development efforts aimed at delivering new mRNA therapeutics and vaccines.
Waters comprehensive and integrated solutions ensure critical quality attributes, including size and polydispersity index (PDI), encapsulation efficiency, particle morphology and stability, and lipid composition are accurately assessed. With robust LC and LC-MS technologies, dynamic light scattering (DLS), and microcalorimetry for the thermodynamics of an LNP sample, Waters solutions deliver powerful performance to increase the analytical sensitivity of your LNP workflow.
The heat map display in DYNAMICS may be programmed to differentiate size distributions and visualize at a glance different degrees of aggregation. Data courtesy Sabin Vaccine Institute.
Simplify LNP analysis with Waters BioAccord LC-MS System with ACQUITY Premier, a fully integrated, compliance-ready platform deployable from discovery to GxP labs.
The ACQUITY Premier System features MaxPeak High Performance Surfaces (HPS) Technology, which offers improvements in separation and detection of metal-sensitive analytes, enabling you to minimize the risk of undetected analytes.
Take single quadrupole performance to the next level with the ACQUITY QDa II Mass Detector that is easy to use, remarkably robust, and complementary to optical detection to confirm and monitor lipid impurities.
Obtain simultaneous measurement of particle size distribution, encapsulated payload concentration, particle concentration, morphology, and aggregation seamlessly and efficiently with Wyatt Technology’s DAWN-MALS, coupled with the ECLIPSE FFF or Waters Arc HPLC System.
Conduct DLS, SLS, and ELS all on one platform with the DynaPro ZetaStar instrument that integrates with Waters Arc HPLC System for automated Zeta potential measurement.
Measure size, polydispersity and Tagg data for hundreds of samples with as little as 4ul per well in standard microplates with the DynaPro DLS Plate Reader, and process with any liquid handler to boost productivity.
Measure and report molecular weight, size, and particle concentration in real time with ultraDAWN real-time MALS (RT-MALS) for rapid feedback on product and process quality.
Gain insight on the overall structure and stability of LNPs with the Nano DSC by monitoring temperature induced changes (melting temperature) of dilute in-solution biomolecules.
Comparison of transfection efficiency into HepG2 cells of LNPs prepared using different methods.
Localization of oxidation on the Dlin-MC3-DMA molecule. Two structures are shown with possible locations for the oxidation, including (left) oxidation of the amine group of the polar head group and (right) epoxidation of one of the double bonds in the fatty acid chain. Below each structure are the low energy (parent ion) and high energy (fragment ion) spectra with matching fragment ions labeled based on in-silico prediction using each structure. Key fragment ions indicative of the location of the oxidation are labeled as A, B, and C. The fragment ion labeled D is the same peak as C but corresponds to an unlikely fragmentation in the epoxidated structure.
Aggregate content in a freshly prepared sample (red) and one which was stored for three months (blue). Top: the light scattering fractograms at 90° scattering angle. Peak 1 is centered around 17 minutes, Peak 2 around 31 minutes. The relative size of the aggregate peak (Peak 2) is significantly larger for the stored sample. Bottom: total particle concentrations, where Peak 2 contains 10 times more particles for the stored sample than for the fresh sample.
(A) Overlay of the effect of heating on Tm. 1st heat scan, red dash and 2nd heat scan, blue line (B) Effect of additives on liposome properties. Black = no cholesterol added, blue = moderate cholesterol percentage incorporated, red = high cholesterol percentage incorporated.
