• Application Note

Acetaminophen in Serum

Acetaminophen in Serum
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of acetaminophen in serum using SymmetryShield columns.

Introduction

Compounds used in this study are:

1: Sulfanilamide (I.S.)

2: Acetaminophen

Experimental

HPLC Method

Column:

SymmetryShield RP8, 3.9 x 150 mm, 5 μm (p/n: WAT200655)

Guard Column:

Sentry Guard Column 3.9 x 20 mm, 5 μm (p/n: WAT200675)

Mobile phase:

20 mM potassium phosphate, pH 3.0/methanol 97:3)

Flow rate:

1.0 mL/min

Injection volume:

50 μL of reconstituted porcine serum extract

Temperature:

30 °C

Detection:

UV @ 220 nm

Results and Discussion

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WA31763.13, June 2003

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