Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Eco Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. GlycoWorks RapiFluor-MS N-Glycan Eco Kits replace DMF with a greener, safer, alternative (DMSO), as a Reagent Solvent for the RapiFluor-MS Reagent Label and remove any other DMF containing solutions. This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Eco Labeling Module, GlycoWorks Eco Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 µm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate. It contains materials to process 96 samples in a 4x24 format. The kit contains: (4) 0.035 ml/vial Rapid PNGaseF Enzyme (4) 0.25 ml/vial Glycoworks Rapid Buffer
(1) 4 vials of 10 mg/vial RapiGest SF Surfactant (1) Intact mAb Mass Check Standard *Shipped in a separate box GlycoWorks RapiFluor-MS Eco Labeling Module: (4) 23 mg/vial RapiFluor-MS Reagent (4) 1 ml/vial Reagent Solvent (Anhydrous DMSO) GlycoWorks RFMS Eco Clean-up Module: (4) 5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7) (1) GlycoWorks HILIC µElution Plate GlycoWorks Sample Collection Module: (15) 1 mL tubes for deglycosylation and labeling reaction (15) 600 µL tube inserts for SPE eluate collection (15) Cap strips with slits for direct injection (1) 1 mL round collection tray (1) Waste tray Extras: (1) 50 mM Ammonium Formate Solution—Glycan Analysis (186007081) (1) ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7μm, 2.1 x 150 mm (186004742) (1) RapiFluor-MS Glycan Performance Test Standard
Suggested Use: Eco-Friendly, Released N-linked Glycan Analysis
Number of Analysis |
96 |
Type |
Sample Preparation Workflow |
Packages per kit |
1 |
Separation Mode |
UPLC/UHPLC/HPLC |
Detection Method |
HRMS, MS, MS/MS, UV |
Storage Condition |
Refer to the storage recommendations of each sub-package |
Tipo de sistema |
LC-MS |
Shelf Life |
Refer to the storage recommendations of each sub-package |
UNSPSC |
41116104 |
Aplicação |
Glicano |
Marca |
GlycoWorks |
Tipo de produto |
Kits para aplicações |