This is an Application Brief and does not contain a detailed Experimental section.
This application brief describes the analysis of alfatoxins in peanuts.
This application brief describes the analysis of alfatoxins in peanuts.
1. Add 5 g of sodium hydroxide to 20 g of homogenized sample, followed by 30 mL of n-hexane.
2. Add 100 mL 60% aqueous methanol and homogenize.
3. Ultrasonicate for 30 minutes.
4. Filter sample through 15 cm filter paper.
5. Take 1 mL aliquot from 60% methanol layer for SPE cleanup.
Instrument: |
Alliance HPLC 2695 System |
Column: |
Symmetry Shield RP18, 4.6 x 150 mm, 5 μm |
Flow rate of iodine: |
0.2 mL |
Flow rate: |
1 mL/min |
Mobile phase: |
A. methanol B. water |
Isocratic gradient: |
35% A: 65% B, for 20 minutes |
Column temperature: |
30 °C |
Derivatization temp.: |
80 °C |
Excitation wavelength: |
365 nm |
Emission wavelength: |
455 nm |
Post-column derivatizaton reagent: |
Dissolve 200 mg iodine in 10 mL methanol, top up 1000 mL with water |
Detector: |
2475 Multi Wavelength Fluorescence |
720002584, April 2008