This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of oligosaccharides using ACQUITY UPLC BEH Amide Columns with acetone as organic modifiers.
Compounds used for this study includes:
1. p-Toluamide
2. Fructose
3. Glucose
4. Sucrose
5. Maltose
6. Maltotriose
7. Maltotetraose
8. Maltopentaose
9. Maltohexahose
10. Maltoheptaose
Column: |
ACQUITY UPLC BEH Amide 2.1 x 50 mm, 1.7 μm |
Part number: |
186004800 |
Mobile phase A: |
80/20 acetone/H2O with 0.05% triethylamine [TEA] |
Mobile phase B: |
30/70 acetone/H2O with 0.05% triethylamine [TEA] |
Flow rate: |
0.17 mL/min |
Gradient: |
5 minute gradient, 80%–60% MeCN with 10 minute re-equilibration |
Injection volume: |
0.7 μL (PLNO) |
Sample concentration: |
1 mg/mL each |
Sample diluent: |
50/50 MeCN/H2O |
Column temperature: |
85 °C |
Strong needle wash: |
20/80 MeCN/H2O (800 μL) |
Weak needle wash: |
75/25 MeCN/H2O (500 μL) |
Seal wash: |
50/50 MeCN/H2O |
Instrument: |
Waters ACQUITY UPLC with ELSD |
Time (min) |
%A |
%B |
---|---|---|
0.00 |
100.00 |
0.00 |
5.00 |
60.00 |
40.00 |
5.01 |
100.00 |
0.00 |
15.00 |
100.00 |
0.00 |
Gain: |
200 |
Pressure: |
40 psi |
Drift tube temperature: |
40 °C |
Nebulizer: |
Cooling |
Data rate: |
10 pps |
Filter time constant: |
Normal |
WA60111, October 2009