• Applikationsbericht

Analysis of Mono-, Di-, and Oligosaccharides Using ACQUITY UPLC BEH Amide Columns with Acetone as Organic Modifiers

Analysis of Mono-, Di-, and Oligosaccharides Using ACQUITY UPLC BEH Amide Columns with Acetone as Organic Modifiers

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of oligosaccharides using ACQUITY UPLC BEH Amide Columns with acetone as organic modifiers.

Introduction

Compounds used for this study includes:

1. p-Toluamide

2. Fructose

3. Glucose

4. Sucrose

5. Maltose

6. Maltotriose

7. Maltotetraose

8. Maltopentaose

9. Maltohexahose

10. Maltoheptaose

Experimental

Chromatographic Conditions

Column:

ACQUITY UPLC BEH Amide 2.1 x 50 mm, 1.7 μm

Part number:

186004800

Mobile phase A:

80/20 acetone/H2O with 0.05% triethylamine [TEA]

Mobile phase B:

30/70 acetone/H2O with 0.05% triethylamine [TEA]

Flow rate:

0.17 mL/min

Gradient:

5 minute gradient, 80%–60% MeCN with 10 minute re-equilibration

Injection volume:

0.7 μL (PLNO)

Sample concentration:

1 mg/mL each

Sample diluent:

50/50 MeCN/H2O

Column temperature:

85 °C

Strong needle wash:

20/80 MeCN/H2O (800 μL)

Weak needle wash:

75/25 MeCN/H2O (500 μL)

Seal wash:

50/50 MeCN/H2O

Instrument:

Waters ACQUITY UPLC with ELSD

Gradient:

Time (min)

%A

%B

0.00

100.00

0.00

5.00

60.00

40.00

5.01

100.00

0.00

15.00

100.00

0.00

ELSD Conditions

Gain:

200

Pressure:

40 psi

Drift tube temperature:

40 °C

Nebulizer:

Cooling

Data rate:

10 pps

Filter time constant:

Normal

Results and Discussion

Featured Products

WA60111, October 2009

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