• Note d'application

Nicotinic Acid

Nicotinic Acid
  • Waters Corporation
Télécharger le PDF Télécharger le PDF

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of nicotinic acid using XTerra RP18 columns.

Introduction

Nicotinic acid has been analyzed in this study.

Experimental

HPLC Conditions

Column:

XTerra RP18 4.6 x 150 mm, 5 μm (p/n: 186000492)

Mobile phase:

At pH 3.0: H2O/ACN/100 mM, NH4COOH, pH 3.0, 85:05:10

At pH 7.0: H2O/ACN/100 mM, NH4HCO3, pH 7.0, 87.5:2.5:10

At pH 10.0: H2O/ACN/100 mM, NH4HCO3, pH 10.0, 90:0:10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile Phase pH

USP Tailing

3.0

1.26

7.0

1.26

10.0

1.26

 

Results and Discussion

Featured Products

WA20738.077, June 2002

Haut de la page Haut de la page