ProteinWorks eXpress Digest Start-up Kit

SKU: 176003696
ProteinWorks eXpress Digest Start-up Kit

ProteinWorks eXpress 消化启动套件 | 176003696


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产品说明

"The ProteinWorks eXpress Digest Start-up Kit simplifies and accelerates the direct digestion of immunopurified plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach. The kit includes pre-measured, lot-traceable reagents, a flexible 96-tube sample collection module, and optimized protocols, offering scientists a standardized, proven route to fast, accurate and precise protein quantification for protein bioanalysis. The ProteinWorks eXpress Digest Start-up Kit contains the eXpress Digest Kit, the ProteinWorks µElution SPE Clean-up Kit, and a murine mAb standard: (96) Denaturant* Cluster Tubes, Cap Mat & Rack for low protein content samples (~0.2 - 1.0 mg total protein per sample) (12) 8-Strip Caps for Cluster tubes (1) Digestion Buffer (1) Trypsin Dissolving Agent (1) Digestion Inactivation Agent (3) Reduction Agent vials. Each vial is enough for 32 samples (3) Alkylation Agent vials. Each vial is enough for 32 samples (1) Trypsin for low protein content samples (~0.2 - 1.0 mg total protein per sample) (1) Intact mAb Mass Check Standard (p/n: 186006552) (1) ProteinWorks µElution SPE Clean-Up Kit (p/n: 186008304) *The denaturant is RapiGest™ SF, an enzyme friendly detergent used to denature proteins prior to digestion. For more information on RapiGest SF, see 720000468en."

规格

  • Number of Analysis

    96

  • Type

    Sample Preparation Workflow

  • Packages per kit

    4

  • Separation Mode

    RPLC

  • Detection Method

    MS/MS

  • Storage Condition

    Refer to the storage recommendations of each sub-package

  • 系统类型

    LC-MS

  • Shelf Life

    Refer to the storage recommendations of each sub-package

  • UNSPSC

    41116104

  • 应用

    肽, 蛋白质

  • 品牌

    ProteinWorks

  • 产品类型

    应用套件

  • Units per Package

    1 pk

产品支持

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ProteinWorks eXpress 消化启动套件

ProteinWorks™样品制备试剂盒与强大可靠的LC-MS方法和技术相结合,使发现和早期开发实验室能够使用代用肽方法实现一致的、可重复的和敏感的蛋白质定量。ProteinWorks™ eXpress 消化试剂盒,ProteinWorks uElution SPE净化试剂盒,以及鼠类mAb标准品都包含在ProteinWorks™ eXpress 消化启动试剂盒中。

ProteinWorks eXpress直接消化试剂盒是一种基于试剂盒的方法,在广泛的单克隆抗体中具有个位数的试剂盒内和试剂盒间的重复性。ProteinWorks试剂盒通过使用一种经过验证的、标准化的、基于试剂盒的技术来保证实验室和数据的一致性,这种技术很容易在分析人员之间和实验室之间进行部署。ProteinWorks eXpress Digest试剂盒简化和规范了使用代用肽技术进行LC/MS蛋白质定量的通常困难的分析前程序。ProteinWorks洗脱SPE试剂盒旨在去除消化后多余的消化试剂、磷脂和其他血浆和血清成分,提高检测灵敏度和系统的适应性。

为确保您收到真正的Waters设备,我们强烈建议您浏览我们的网站或目录,您可以在这里选购实验室设备,以满足您实验室的确切需求,并以最佳市场价格获得我们的设备。该网站还让您浏览整个Waters设备的选择,并确认您收到的设备是正宗的。

您可能还对HILIC QC参考资料感兴趣,该资料是作为这种类型分离的起点而创建的。它是一种由四种成分组成的混合物,由一种未被保留的空隙标记物、一种极性中性化合物和两种极性碱性化合物组成,专门作为ACQUITY UPLC BEH HILIC色谱柱的批量测试标准。HILIC质控标准物质的保质期为12个月,可方便地存放在实验室中,温度为4摄氏度。

如果我的色谱发生变化,应该如何应对?

色谱变化的两个最常见的原因是:溶液导致色谱柱变质,或者有东西卡在色谱柱上需要被洗掉。您可以通过仔细监测和改变系统中液体的pH值和压力来帮助防止退化,并使用硅胶色谱柱。为了去除粘结的有机物,我们亦建议用与色谱柱相同极性的溶剂进行清洗。高浓度盐的冲洗会消除离子交换色谱柱中的绝大多数污染物。