Oligonucleotide Purification Columns and Consumables

For preparative columns with smaller aspect ratios, high pressure slurry packing with small particles often fails to achieve the desired bed density found in well-designed analytical columns. Excessive axial compression at the inlet can result in particle breakage, channeling, and reduced bed permeability. Thoughtfully designed OBD columns offer significant benefits, including longer column lifetime, improved efficiency, better peak shape, and lower back pressure.

Nucleic acids, including oligonucleotides are polyanionic (negatively charged) and as such readily adsorb metal oxide surfaces in stainless steel columns. Waters XBridge Premier Oligonucleotide BEH C₁₈OBD Columns are built with hardware components modified to have an inert hybrid organic/inorganic silica-based surface chemistry that greatly reduces non-specific adsorption (NSA) of nucleic acids, thus enabling enhanced recovery and improved reproducibility for certain compounds that need to be isolated from a drug substance for further study. XBridge Premier Oligonucleotide BEH C₁₈ OBD Columns also eliminate the need for column passivation/sample conditioning so you can start your fraction collection runs without delay. 

Waters batch-certified Bridged Ethyl Hybrid (BEH) technology for oligonucleotides ensures extra performance, reproducibility, and scale-up for purification. High temperature and high pH are prevalent strategies for minimizing secondary interactions in oligonucleotide separation and purification. BEH particle technology withstands harsh conditions such as high pH, temp, and additives that usually are required for oligonucleotide analysis.

Batch-tested and QC-certified XBridge Oligonucleotide OBD Prep Columns provide extra assurance for reproducibility and method transfer, and support emerging purification needs for standard and new chemically diverse siRNAs, ASOs, and oligonucleotide reagents. Each batch of Oligonucleotide BEH C₁₈ sorbent is QC tested and selected using Waters MassPrep Oligonucleotide Standard and a stringent resolution and peak tailing assessment from a triethylammonium acetate IP-RP separation. These carefully examined sorbents are available in 2.5 and 5 µm particle sizes as well as 130 and 300 Å pore sizes to give oligonucleotide chemists a range of versatile options to purify a variety of synthesis products.

XBridge and XBridge Premier Oligonucleotide OBD Prep Columns are packed with Waters BEH (Bridge Ethyl Hybrid) C₁₈ hybrid silica particles and demonstrate remarkable column longevity under high pH and temperature conditions while maintaining outstanding separation performance. With careful care, Oligonucleotide BEH C₁₈ Columns have been known to exceed 1,0000 injections without loss of efficiency or retention. 

In preparative purifications, loading capacity is influenced by multiple parameters. Column efficiency, pore size, particle size, and method parameters play significant roles. Larger pore size columns allow for better mass transfer and sharper peak shapes of larger analytes. With their reduced surface area, they can exhibit lower loading capacity. Waters XBridge Oligonucleotide OBD Prep Columns are available in different pore sizes and a wide range of column I.D.s for predictable scalability and improved loading capacity.

High purity and quality oligonucleotide purification is critical to minimize the safety and efficacy concerns related to unwanted impurities in toxicological studies. The high-efficiency 2.5 µm XBridge Oligonucleotide OBD Prep Columns facilitating isolation of early and co-eluting impurities while maintaining chromatography profile without compromising high recovery.