• Application Note

Separation of a Tryptic Digest of Bovine Hemoglobin

Separation of a Tryptic Digest of Bovine Hemoglobin
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates separation of a tryptic digest of bovine hemoglobin.

Introduction

The compound used in this study is Bovine haemoglobin.

Experimental

Column:

BioSuite PA-A and PA-B Columns (2.1 x 150 mm)

Eluent A:

0.02% TFA in water

Eluent B:

0.016% TFA in acetonitrile

Gradient:

0–56% B in 70 minutes

Flow rate:

0.16 mL/min

Injection volume:

15 μL

Temperature:

40 °C

Detection:

UV 220 nm

Results and Discussion

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WA31791, June 2003

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