Viral vector-based gene therapies are large drug product formulations comprised of protein and nucleic acid components containing over 200,000 atoms. Waters liquid chromatography (LC) combined with optical detection (UV, MALS) and mass spectrometry (MS) provide the analytical means to accurately characterize and quantify these heterogeneous structures and their attributes, at both component and intact levels. Routine measurements include viral titer, aggregate/impurities analysis, viral protein ratios, peptide mapping & modification analysis, encapsidation efficiency, and genome integrity.
Webinar: Small Particle Low Adsorption SEC Columns for Fast and Efficient Characterization of AAV via SEC-MALS