This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the analysis of brompheniramine using XTerra Columns.
In this study the compound analyzed is brompheniramine.
Column: |
XTerra RP18 4.6 x 150 mm, 5 µm |
Part number: |
186000492 |
Mobile phase: |
pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 45:45:10 pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 50:40:10 pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 50:40:10 |
Flow rate: |
1.0 mL/min |
Injection volume: |
5 µL of 250 µg/mL |
Temperature: |
30 °C |
Detection: |
UV @ 270 nm |
Instrument: |
Alliance 2695, 2996 PDA |
Mobile Phase pH |
USP Tailing |
---|---|
3.0 |
1.22 |
7.0 |
1.37 |
10.0 |
1.18 |
WA20738.018, June 2002