Methylnicotinic Acid

Column:

XTerra RP₁₈ 4.6 x 150 mm, 5 µm

Part number:

186000492

Mobile phase:

pH 3.0: H₂O/ACN/100 mM NH₄COOH, pH 3.0 85:05:10

pH 7.0: H₂O/ACN/100 mM NH₄HCO₃, pH 7.0 85:05:10

pH 10.0 H₂O/ACN/100 mM NH₄HCO₃, pH 10.0 90:0:10

Flow rate:

1.0 mL/min

Injection volume:

5 µL of 250 µg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA