This is an Application Brief and does not contain a detailed Experimental section.
This application brief details on the analysis of antipyrine and its metabolites using Symmetry Columns.
The compounds analyzed in this study are:
Column: |
Symmetry Shield RP8, 4.6 x 50 mm, 5µm |
Part number: |
186000224 |
Mobile phase: |
0.1% glacial acetic acid/methanol 85:15 |
Flow rate: |
1.0 mL/min |
Injection volume: |
5 µL solution with antipyrine (240 ng) and 4-hydroxyantipyrine (29 ng) |
Detection: |
UV @ 254 nm |
WA31763.31, June 2003