• Application Note

Anilines - 4.0 Min Gradient

Anilines - 4.0 Min Gradient
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of anilines.

Introduction

The compounds used in this study are –

  1. Caffeine
  2. Aniline
  3. N-Methylaniline
  4. 2-Ethylaniline
  5. 4-Nitroanisole
  6. N,N-Dimethylanine

Experimental

Conditions

Column:

Xterra MS C18 4.6 x 20 mm, 3.5 μm

Part number:

186001891

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3, pH 10.0

Flow rate:

3.0 mL/min

Injection volume:

5.0 μL

Sample concentration:

20 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance HT 2795, 2996 PDA

Gradient

Results and Discussion

Featured Products

WA20738.011, June 2002

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