• Application Note

Chlordiazepoxide and Metabolites in Serum

Chlordiazepoxide and Metabolites in Serum
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of chlordiazepoxide and metabolites in serum.

Introduction

The compounds used in this study are –

  1. Norchlordiazepoxide
  2. Oxazepam
  3. Nordazepam
  4. Chlordiazepoxide
  5. Demoxepam (I.S.)

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm

Guard column:

Sentry Guard Column, 3.9 x 20 mm, 5 μm

Part number:

Column - WAT046980, Guard - WAT054225

Mobile phase:

20 mM potassium phosphate, pH 7.0/acetonitrile/methanol 56:21:23

Flow rate:

1.0 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Detection:

UV @ 240 nm

Results and Discussion

wa31763.54-t1

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

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WA31763.54, June 2003

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