• Application Note

Codeine and Its Glucuronide Metabolite in Human Urine

Codeine and Its Glucuronide Metabolite in Human Urine
  • Waters Corporation
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For forensic toxicology use only.

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of codeine and Its glucuronide metabolite in human urine using Symmetry C18 columns.

Introduction

Compounds used in this study are:

1. Ranitidine (I.S.)

2. Codeine-6-Glucuronide (C-6-G)

3. Codeine

Experimental

HPLC Method

Column:

Symmetry C18, 2.1 x 150 mm, 3.5 μm (p/n: 186000174)

Guard column:

Symmetry C18, 2.1 x 10 mm, 3.5 μm (p/n: 186000169)

Mobile phase:

Methanol/Acetonitrile/0.05% TFA in water 5:5:90

Flow rate:

0.3 mL/min

Injection volume:

80 μL urine extract

Temperature:

30 °C

Detection:

UV @ 220 nm (0.04 AUFS)

Results and Discussion

Featured Products

WA31763.63, June 2003

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