XBridge Columns

XBridge Columns

Particle stability and performance for methods development

Particle stability and performance for methods development

Withstanding extreme operating conditions like high pH and high temperatures is essential for methods development. XBridge HPLC and UHPLC Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology and are built to withstand high pH, high temperatures, and other extreme operating conditions like volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis.

XBridge HPLC and UHPLC Columns are available in a broad range of sorbent particles and sizes: XBridge eXtended Perfomance (XP) 2.5 µm Columns for rapid analytical UHPLC separations; 3.5 and 5 µm HPLC columns for reliable analytical method development; and 5 and 10 µm optimum bed density (OBD) preparative columns for purification chromatography.

XBridge BEH HPLC and UHPLC Column family, ideal for method development. XBridge Columns
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Unwanted interactions between analytes and metal ions?

XBridge Premier Columns with MaxPeak HPS technology mitigate interactions with stainless-steel column hardware.

Specifications

Overview

  • Provides high pH stability and chemical resistance for reliability and longevity
  • Enables maximum method development flexibility
  • Available in a range of BEH column chemistries: C18, C8, Shield RP18, Phenyl, HILIC, and Amide fully scalable 2.5 XP, 3.5, 5 μm analytical, and 10 μm OBD preparative particle sizes and column formats
  • Application-specific columns for proteins, peptides, oligonucleotides, and glycan analysis
  • Prolongs column lifetimes with optional XBridge VanGuard Cartridges

Recommended Use: For methods development flexibility with improved analyte peak shape and recovery, even under extreme operating conditions.

XBridge Column Featured Technologies

XBridge Column Featured Technologies
Ethylene Bridged Hybrid (BEH) Technology
  • Rugged hybrid organic/inorganic base particle
  • Improved peak shape for bases-low silanol activity
  • Wider operating pH range for method development flexibly
  • Mechanical strength for maximum column efficiency at UHPLC operating pressures

XBridge BEH Column Characteristics

XBridge BEH Column Characteristics
XBridge BEH C18 Columns
  • General purpose reversed-phase C18 column
  • Superior chemical stability pH 1–12
  • Elevated operating temperature, up to 90 °C
XBridge BEH Shield RP18 Columns
  • Alternative selectivity to traditional alkyl chain C18 columns
  • Improved basic analyte peak shape near neutral pH
  • Compatible with 100% aqueous mobile phases
XBridge BEH Amide Columns
  • General purpose HILIC column
  • Ideal chemistry for the separation of carbohydrates/saccharides
  • Excellent chemical stability pH 2–11
  • Elevated operating temperature, up to 90 °C
XBridge BEH C8 Columns
  • General purpose reversed-phase column
  • Ideal chemistry for the separation of strongly hydrophobic compounds
  • Excellent chemical stability pH 1–12
  • Elevated operating temperature, up to 90 °C
XBridge BEH Phenyl Columns
  • Alternative selectivity to C18 chemistries
  • Unique selectivity for aromatic compounds
  • Excellent chemical stability pH 1–12
  • Elevated operating temperature, up to 80 °C
XBridge BEH HILIC Columns
  • General purpose HILIC column
  • Alternative selectivity to BEH Amide chemistries
  • Unique selectivity for strong polar basic compounds
  • Excellent chemical stability pH 1–9

XBridge HPLC and UHPLC Application-Specific Column Characteristics

XBridge HPLC and UHPLC Application-Specific Column Characteristics
XBridge BEH Glycan Columns
  • Suited for analyzing released and 2-aminobenzamide (2-AB) labeled N-linked glycans from various biotherapeutics

Try XBridge BEH Glycan Columns

XBridge BEH Oligonucleotide Columns
  • Suited for the analysis of detritylated synthetic oligonucleotides using ion-pair, reversed-phase chromatography

Try XBridge BEH Oligonucleotide Columns

XBridge BEH Peptide Columns
  • Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
  • Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
  • Two pore sizes (130 Å and 300 Å) for a wide range of peptides

 Try XBridge BEH Peptide Columns

XBridge BEH Protein & Protein SEC Columns
  • Suited for the separation of proteins by size exclusion or by reversed-phase chromatography, maximizing recovery and minimizing protein carryover
  • Deliver high resolution size-exclusion chromatography (SEC) separations of mAb, aggregates, monomers, and fragments in less time than many traditional SEC columns

Try XBridge BEH Protein & Protein SEC Columns

Built with BEH for mechanical strength and chemical stability

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

Highest pH stability

XBridge HPLC and UHPLC Columns offer higher mobile-phase pH stability than any other commercially available chromatographic phase, making it ideal for method development. Unlike approaches that claim high pH resistance, the pH stability of these columns is created by utilizing proprietary surface modifications and is incorporated into the particle synthesis process.

The columns were tested under accelerated pH 10 stability test conditions against some of the most popular chromatographic phases that claim extended high pH stability. The C18 columns showed very little degradation in chromatographic performance, exceeding the performance of the best silica column by more than 1000%.

Preparative separation performance

Waters innovative Optimum Bed Density (OBD) Preparative design ensures direct scalability that is independent of particle size. The combination of the revolutionary OBD format with the BEH base particle delivers the most stable, efficient, and reproducible preparative LC columns available. XBridge OBD Preparative Columns for LC users benefit from high pH and temperature stability for increased mass loading for basic compounds.

Protect your column without compromise

Don’t take chances. Complex sample matrices encountered in pharmaceutical, natural product, environmental, and industrial chemical analysis can prematurely shorten analytical UHPLC and/or HPLC column lifetime and degrade chromatographic performance. Using guard cartridges like VanGuard Protection products improve analytical performance by removing particulate and chemical contamination that may be present in the mobile-phase stream—without compromising chromatographic performance.

Stay compliant with smart column options

Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (3 µm particles) used with Alliance iS HPLC System that is optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.

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Related

XBridge Premier Columns
Experience XBridge Premier HPLC and UHPLC Columns, designed to operate under extreme temperatures and mobile-phase pH while mitigating unwanted analyte/surface interactions.

MaxPeak Premier Columns
Experience the highest level of chromatographic performance, flexibility, and assurance while mitigating unwanted analyte/surface interactions with MaxPeak Premier Columns.

Arc Premier System
Confidently produce the most accurate, reliable, and reproducible data. The Arc Premier System provides right first time robust results from the very first injection, reliably and easily.

VanGuard Column Protection
Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

Offers and Discounts

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XBridge Columns

XBridge Columns from Waters are designed to deliver reliable, high-performance separations across a wide range of applications. Engineered with exceptional durability and chemical stability, XBridge Columns provide consistent and reproducible results, making them ideal for demanding workflows in pharmaceutical development, biopharmaceutical characterization, food safety, environmental testing, and more. Whether you"re working with complex mixtures or challenging conditions, XBridge Columns ensure precision and confidence in every analysis.

At the heart of XBridge Columns is the proprietary BEH (bridged ethyl hybrid) particle technology, which combines the benefits of silica and hybrid organic/inorganic materials. This innovative design enhances the columns" stability under extreme pH, temperature, and solvent conditions, ensuring long-lasting performance even in harsh environments. BEH particles are available in a variety of particle sizes, from sub-2 µm for ultra-high resolution to larger particles suited for preparative or legacy HPLC workflows, providing flexibility to match your specific requirements.

XBridge Columns support a wide range of separation techniques, including reversed-phase, hydrophilic interaction chromatography (HILIC), and ion exchange. Reversed-phase chemistries such as C18, C8, and phenyl provide excellent retention and selectivity for hydrophobic compounds, while HILIC chemistries excel in separating polar analytes. Ion-exchange options enable the separation of charged molecules, such as proteins and peptides, with outstanding resolution. This variety ensures that XBridge Columns can handle virtually any analytical challenge, offering unmatched versatility for method development and routine analyses.

The durability of XBridge Columns extends beyond their chemical stability. They are built to withstand high pressures, ensuring compatibility with modern UHPLC systems while maintaining peak performance in traditional HPLC setups. This flexibility makes XBridge Columns a trusted solution for laboratories looking to upgrade their workflows without sacrificing reliability or resolution. Additionally, the columns" consistent performance across different particle sizes allows seamless scalability from analytical to preparative applications, ensuring efficiency at every stage of your process.

XBridge Columns also feature excellent peak shape and minimal tailing, even for challenging analytes such as basic compounds. This is achieved through their advanced packing technology and optimized stationary phases, which reduce secondary interactions and enhance chromatographic performance. These qualities make XBridge Columns an invaluable tool for achieving high resolution and reproducibility, even in the most complex separations.

Waters provides robust support for XBridge Columns, with compatibility across a range of systems, including Waters ACQUITY UPLC, HPLC, and third-party instruments. Paired with intuitive software solutions like Empower and MassLynx, these columns offer seamless integration into your laboratory"s workflow. Whether you"re optimizing a new method or performing routine quality control, XBridge Columns deliver the precision and reliability required for success.

Elevate your laboratory"s capabilities with XBridge Columns, where innovative technology meets practical application. Experience superior performance, durability, and versatility across a spectrum of chromatographic challenges, ensuring confidence in every result.

FAQs About XBridge Columns

1. What applications are XBridge Columns best suited for?
XBridge Columns are highly versatile and suitable for a wide range of applications, including pharmaceutical development, biopharmaceutical characterization, food safety testing, and environmental analysis. These columns excel in handling complex mixtures, such as impurities or metabolites, and are particularly effective for method development, stability studies, and quality control.

2. How does BEH particle technology enhance the performance of XBridge Columns?
BEH (bridged ethyl hybrid) particle technology combines the strengths of silica and organic/inorganic hybrid materials, delivering unmatched chemical and mechanical stability. This allows XBridge Columns to operate effectively across a wide pH range, at elevated temperatures, and under harsh solvent conditions. BEH particles also reduce column degradation and extend lifespan while providing consistent, high-resolution separations. Their optimized surface chemistry minimizes secondary interactions, improving peak shape and enhancing the separation of challenging analytes like polar and basic compounds.

3. What separation techniques can XBridge Columns be used for?
XBridge Columns support a variety of separation techniques, including reversed-phase, hydrophilic interaction chromatography (HILIC), and ion-exchange chromatography. Reversed-phase chemistries such as C18 and C8 are ideal for hydrophobic analytes, while HILIC chemistries excel in separating polar compounds. Ion-exchange options are designed for charged molecules, such as proteins and peptides, offering high resolution and selectivity. This versatility ensures that XBridge Columns can handle diverse sample types and analytical challenges with precision and efficiency.

4. What is the pH range compatibility for XBridge Columns?
XBridge Columns are compatible with an extended pH range of 1 to 12, depending on the specific stationary phase. This wide range allows users to work with both acidic and basic conditions, making the columns highly versatile for various applications. The exceptional pH stability provided by BEH particle technology ensures consistent performance, even in workflows requiring extreme pH conditions.