RapiGest SF Surfactant

RapiGest SF Surfactant

Enhance the quality of in-solution enzymatic protein digestions

Enhance the quality of in-solution enzymatic protein digestions

Labs around the globe are trying to simplify digestion protocols and improve throughput of analysis. Waters patented RapiGest SF Surfactant was designed with your lab’s efficiency in mind, significantly decreasing proteolytic digestion time, and enabling same-day digestions with mass spectrometry (MS) compatibility.

RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. This patented anionic surfactant accelerates the in-solution production of peptides generated by proteases, such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F. Many hydrophobic proteins are resistant to proteolysis because their cleavage sites are inaccessible to endoproteases. RapiGest SF facilitates the digestion of proteolytically resistant proteins and increases the solubility of hydrophobic proteins and peptides. RapiGest SF is an MS-compatible mild protein denaturant that helps solubilize and unfold proteins, making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes like conventional denaturants. This enables use of lesser amounts of expensive endoproteases.

RapiGest SF Protein Digestion Surfactant. RapiGest SF Surfactant
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Specifications

Overview

  • Compatible with liquid chromatography (LC) and MS analysis
  • Enables same-day digestions and analysis
  • Minimal or no post-digestion sample preparation is required
  • Improves efficiency and speed of digestion
  • Simplifies digestion protocols and improves throughput of analysis
  • Does not inhibit enzyme activity, unlike conventional denaturants
  • Allows use of lesser amounts of expensive endoproteases because it is not disruptive of endoprotease activity

Recommended Use: For improving protein sequence coverage and in-solution digestion protocols.

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How RapiGest SF Surfactant works

RapiGest SF Surfactant is a mild denaturant that solubilizes and unfolds the substrate protein, making it more amenable to cleavage. Mild protein denaturation opens the protein structure and exposes the proteolytic sites to enzymatic cleavage. You can accomplish this during a more complex denaturation of globular proteins by heating in a RapiGest SF solution at elevated temperatures.

Compatible with MS

RapiGest SF is an acid labile surfactant that undergoes hydrolysis in acidic conditions. This unique feature allows you to utilize it to remove the surfactant from solutions when desired. Acidic conditions break down RapiGest SF to non-interfering byproducts, therefore peptide ionization is not suppressed.

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RapiGest SF Surfactant

RapiGest SF Surfactant is a groundbreaking solution that is made to significantly enhance the efficiency and effectiveness of in-solution enzymatic protein digestions. Laboratories around the world are continually seeking ways to streamline digestion protocols and improve the throughput of analysis, and RapiGest SF stands out as a tool for achieving these goals. This patented anionic surfactant, developed by Waters, is engineered to dramatically decrease proteolytic digestion time, enabling same-day digestions with full compatibility for mass spectrometry (MS) applications.

At the heart of RapiGest SF's functionality is its ability to accelerate the production of peptides during enzymatic digestions. Proteases such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F benefit immensely from the presence of RapiGest SF, as it facilitates the cleavage of proteolytically resistant proteins. Many hydrophobic proteins pose a challenge during digestion due to their structural resistance; their cleavage sites are often buried within the protein, making them inaccessible to endoproteases. RapiGest SF effectively addresses this challenge by increasing the solubility of these hydrophobic proteins and peptides, allowing for more complete and efficient digestion.

What sets RapiGest SF apart from conventional denaturants is its mild nature and MS compatibility. Unlike traditional denaturants that can disrupt or inhibit the activity of proteolytic enzymes, RapiGest SF unfolds proteins in a way that maintains the integrity and activity of these enzymes. This feature is particularly advantageous as it allows for the use of smaller quantities of expensive endoproteases without compromising the quality of digestion. The surfactant acts as a mild protein denaturant, solubilizing, and unfolding substrate proteins to expose their proteolytic sites, making them more accessible to enzymatic cleavage.

The versatility of RapiGest SF extends to its compatibility with liquid chromatography (LC) and MS analysis. This surfactant is acid-labile, meaning it undergoes hydrolysis under acidic conditions. This unique property allows researchers to easily remove the surfactant from solutions when desired, as the acidic environment breaks down RapiGest SF into non-interfering byproducts. As a result, peptide ionization is not suppressed, ensuring that the mass spectrometry results are accurate and reliable.

RapiGest SF also excels in simplifying digestion protocols and improving the overall throughput of analysis. By enabling same-day digestions and minimizing the need for extensive post-digestion sample preparation, this surfactant streamlines workflows and reduces the time required to achieve high-quality results. Laboratories can thus enhance their productivity and efficiency without sacrificing the accuracy of their proteomic analyses.

RapiGest SF Surfactant is an essential component for any laboratory seeking to improve protein sequence coverage and in-solution digestion protocols. Its ability to enhance the solubility and cleavage of proteolytically resistant proteins, combined with its mild, enzyme-friendly denaturation properties, makes it a superior choice for facilitating efficient and reproducible protein digestions.

With RapiGest SF, laboratories can achieve faster, more reliable results, all while reducing costs associated with enzyme usage and complex sample preparation. Whether your focus is on pharmaceutical research, clinical diagnostics, or any other field requiring precise proteomic analysis, RapiGest SF Surfactant provides the efficiency and effectiveness needed to advance your work.