• Application Note

Amphetamines in Human Urine

Amphetamines in Human Urine
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of amphetamines in human urine using Symmetry Columns. 

Introduction

The compounds analyzed in this study are:

  1. Ephedrine
  2. Amphetamine 
  3. Methamphetamine 

Experimental

HPLC Method

Column:

Symmetry Shield RP18, 3.9 x 150 mm, 5 µm

Guard column:

Symmetry Guard Column, 3.9 x 20 mm, 5 µm

Part numbers:

Column - 186000108, Guard - 186000107

Mobile phase:

0.1% TFA in Water/Acetontrile 90:10

Flow rate:

1.0 mL/min

Injection volume:

50 µL

Temperature:

30˚ C

Detection:

UV @ 214 nm (0.14 AUFS)

Results and Discussion

Chromatogram of A) Spiked Urine, B) Blank Urine. 

Featured Products

WA31763.27, June 2003

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