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Trimethoprim in Rat Plasma

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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the analysis of Trimethoprim in Rat Plasma using Symmetry Columns.

Introduction

The compound analyzed in this study is Trimethoprim.

Experimental

HPLC Method

Column:	XTerra MS C₁₈ 2.1 x 30 mm, 3.5 µm
Part number:	186000398
Mobile phase A:	1.0% NH4OH
Mobile phase B:	ACN
Isocratic mobile phase composition:	40% A; 60% B
Flow rate:	0.2 mL/min
Injection volume:	30 µL
Detection:	MS ESI+
Instrument:	Alliance 2790, Micromass Quattro Ultima
Ion source:	ESI+
Source temperature:	150 °C
Gas cell:	1.5e^-3 mbar, 25 eV
Desolvation temperature:	350 °C
Cone gas flow:	150 L/hr
Drying gas flow:	600 L/hr
Cone voltage:	20V