Maximum Pressure |
6000 psi (415 Bar) |
Particle Shape |
Spherical |
Particle Size |
10 µm |
Endfitting Type |
Waters |
Format |
Column |
System |
HPLC |
USP Classification |
L19 |
Inner Diameter |
6.5 mm |
Length |
300 mm |
UNSPSC |
41115709 |
Application |
Carbohydrate |
Brand |
Sugar-Pak |
Product Type |
Columns |
Units per Package |
1 pk |
Sugar-Pak I Column, 10 µm, 6.5 mm X 300 mm, 1/pk
Ideal for the separation of monosaccharides, polyols, and sugar alcohols via a strong cation-exchange mechanism, the Waters Sugar-Pak I column is an important piece of lab equipment for any research institute. Whether you need to inspect the quality of commercial sugar products or perform analysis of sugar products and process streams in beet, cane, and starch hydrolysis processing plants, the Waters Sugar-Pak I 10 µm column will be suitable for your requirements.
The 6.5mm (I.D.)x 300mm Waters Sugar-Pak I Column is packed with a microparticle cation-exchange gel in calcium form. Based on a sulfonated styrene-divinylbenzene polymer, the resin provides pH stability by means of a calcium counterion.
Successfully separate glucose, fructose, maltose, and maltotriose from the higher oligomers found in corn syrups with the Sugar-Pak I column. It allows the course of alcoholic fermentation to be followed by monitoring the reduction of fermentable sugars and the production of alcohol.
Separate larger sugars as well as disaccharides reliably using the Waters Sugar-Pak column according to molecular weight. It also enables you to conduct many other useful separations where a variety of monosaccharides or sugar alcohols require separation and analysis. These include fruit juices, non-nutritive sweeteners containing sorbitol or mannitol, cell, and cell hydrolyzates.
The Waters Sugar-Pak column is compatible with the Sugar-Pak Guard-Pak Insert, 10 µm, 10/pk that prolongs the life of the Sugar-Pak column.
How Do You Care For And Use The Sugar-Pak I Columns?
Liquid chromatography columns have a finite life influenced by their care and use, number of injections, sample and solvent cleanliness, frequency of solvent changeover, and handling and storage procedures (among other factors). If a change is observed in the retention of a particular compound, the resolution between two compounds, or the peak shape, you should take immediate steps to determine the reason for the changes. Until you have made this determination, you must not rely upon the results of any separation using the column. Follow generally accepted procedures for quality control and methods development when using these columns. Note: Before running the first analysis on your new column, perform the test sample separation given in the test conditions section.
What Steps Should Be Followed Before Using The Sugar-Pak I Column For The First Time?
Before using your new Sugar-Pak I Column for the first time, it is recommended that the following procedure be observed: First, you should connect the column. Next, you should set the flow rate at 0.2 mL/min until the column temperature reaches 70 °C. Third, you should wait for the column temperature to reach 70 °C, after which you should increase the flow rate to 0.6 mL/min in steps of 0.1 mL/min. Finally, wait for the backpressure to stabilize between each 0.1 mL/min increase.