• Application Note

Nucleotide Triphosphates (dNTP)

Nucleotide Triphosphates (dNTP)
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of nucleotide triphosphates using XTerra MS C18 columns.

Introduction

Compounds used in this study are:

1. Deoxycytidine 5’-triphosphate

2. Thymidine 5’-triphosphate

3. Deoxyguanosine 5’-triphosphate

4. Adenosine 5’-triphosphate

Experimental

HPLC Conditions

Column:

XTerra MS C18 4.6 x 30 mm, 2.5 μm (p/n: 186000600)

Mobile phase A:

50 mM Triethylammonium acetate, pH 7.0, 2 mM EDTA

Mobile phase B:

ACN

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Detection:

UV @ 260 nm

Temperature:

20 °C

Instrument:

Alliance 2695, 2996 PDA

Gradient

Results and Discussion

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WA20738.081, June 2002

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