• Application Note

Sulfa Drugs – 2.1 x 20 mm Intelligent Speed Separation

Sulfa Drugs – 2.1 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of sulfa drugs using Intelligent Speed Separation Symmetry columns.

Introduction

Compounds used in this study are:

1. Sulfadiazine

2. Sulfathiazole

3. Sulfamerazine

4. Sulfadimidine

5. Sulfamethoxazole

6. Sulfisoxazole

7. Sulfadimethoxine

Experimental

HPLC Method

Column:

Symmetry C18, 2.1 x 20 mm, 3.5 μm IS (p/n: 186002066)

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in MeOH

Flow rate:

0.6 mL/min

Injection volume:

10 μL

Temperature:

30° C

Detection:

UV @ 270 nm

Gradient

Results and Discussion

WA31763.156, June 2003

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