• Application Note

Peptides - Alberta Peptide Mixture 

Peptides - Alberta Peptide Mixture 

  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of Alberta peptide mixture using Symmetry C18.

Introduction

The Alberts peptide mixture includes:

Ac-Arg-Gly-x-x-Gly-Leu-Gly-Leu-Gly-Lys-amide
where x-x=
S1 Ala=Gly with free N alpha amino group
S2 Gly-Gly
S3 Ala-Gly
S4 Val-Gly
S5 Val-Val

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm (p/n: WAT046980)

Mobile phase A:

0.1% TFA in water

Mobile phase B:

0.1% TFA in acetonitrile

Flow rate:

0.8 mL/min

Injection volume:

9 μL of peptide mixture; lyophilized sample dissolved in 1 mL mobile phase

Temperature:

35° C

Detection:

UV @ 214 nm

USP tailing factors:

1. 1.3

2. 1.2

3. 1.2

4. 1.2

5. 1.3

Gradient

Time (min)

%A

%B

0

90

10

30

60

40

35

40

60

Results and Discussion

Featured Products

WA31763.121, June 2003

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