Trimethoprim in Rat Plasma

Column:

XTerra MS C₁₈ 2.1 x 30 mm, 3.5 µm

Part number:

186000398

Mobile phase A:

1.0% NH4OH

Mobile phase B:

ACN

Isocratic mobile phase composition:

40% A; 60% B

Flow rate:

0.2 mL/min

Injection volume:

30 µL

Detection:

MS ESI+

Instrument:

Alliance 2790, Micromass Quattro Ultima

Ion source:

ESI+

Source temperature:

150 °C

Gas cell:

1.5e^-3 mbar, 25 eV

Desolvation temperature:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

20V