• Application Note

Analysis of Caffeine Metabolites Using XBridge Phenyl

Analysis of Caffeine Metabolites Using XBridge Phenyl
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of caffeine metabolites using XBridge Phenyl columns.

Introduction

Experimental

Test Conditions

Columns:

XBridge Phenyl, 4.6 x 100 mm, 3.5 μm p/n: 186003334

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Sample:

Caffeine (10 μg/mL), Theobromine (10 μg/mL),

1-Methyl xanthine (10 μg/mL),

1,3-Dimethyluric Acid (10 μg/mL),

1,7-Dimethyl xanthine (10 μg/mL),

1,7-Dimethyluric Acid (10 μg/mL) in H2O/NH4HCO3 (90/10)

Column temp.:

30 °C

Sampling temp.:

15 °C

Detection:

UV @ 280 nm

Sampling Rate:

5 points/sec

Filter Response:

0.2

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time(min)

%A

%B

%C

0

89

1

10

9

66

24

10

10

89

1

10

20

89

1

10

Results and Discussion

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WA60187, June 2007

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