Preparative HPLC Columns
Increase throughput with preparative productivity and predictability of small molecules, proteins, peptides, and nucleic acid-based analytes.
As a scientist working on the research, development and commercialization of diagnostic or therapeutic drugs it can be challenging to create methods that are robust, scalable and provide high yield and purity across various preparative LC platforms.
As your lab works to achieve fast, efficient, lab-scale separations and greater throughput, Waters Preparative HPLC Columns can help you attain predictable performance and easily scale from UPLC, UHPLC, or HPLC screening to lab-scale purification. With the proprietary Optimum Bed Density (OBD) design of Waters Preparative HPLC Columns, you can ensure consistency in column-to-column performance and increase productivity through higher recoveries and longer column lifetimes – making lost samples, repeat purification runs, and poor scalability from small to large volume columns a thing of the past.
Select XBridge and XSelect OBD Preparative Columns are now available in MaxPeak HPS Technology hardware in 2.5, 3.5, and 5 µm particle sizes. MaxPeak Premier OBD Preparative Columns prevent undesired compound/surface interactions, leading to poor peak shapes/purities, low sensitivity, and target sample loss.
Preparative HPLC Columns
Specifications
Overview
- Achieve fast, efficient, lab-scale separations for greater throughput
- Directly scale from analytical UPLC, UHPLC, or HPLC screening to lab-scale purification
- Select robust chromatographic particles designed for purification
- Increase productivity through higher recoveries and longer column lifetimes
Recommended Use: For laboratory-scale HPLC purification.
Waters OBD Preparative Columns
-
High pH and temperature stability -
Increased mass loading for basic compounds
-
Increased column lifetime with OBD technology
-
Direct scalability from analytical to prep
-
Benefits of BEH particle technology
-
Pore sizes: 130 Å and 300 Å
-
Particle sizes: 2.5 µm, 3.5 µm, 5 µm, and 10 µm
-
Application-specific chemistries for: proteins, peptides, and oligonucleotides
-
Simplifies method transfer between laboratories
-
QC-tested for peptide, protein and oligonucleotide applications
-
Available with MaxPeak Premier HPS Technology
-
Excellent pH stability
-
Designed for selectivity
-
Improved throughput with high mass loading
-
Increased column lifetime with OBD technology
-
Scalability from larger to smaller preparative columns
-
Benefits of Charged Surface Hybrid (CSH) technology
-
Pore sizes: 130 Å
-
Particle sizes: 3.5 µm, 5 µm and 10 µm
-
Application-specific chemistries for: peptides
-
Reduces solvent consumption and fraction volumes
-
Ideal for rapid method development
-
QC batch-tested with complex protein digest
-
Available with MaxPeak Premier HPS Technology
-
Low pH stability
-
High mass loading
-
Easy scale-up from analytical to preparative
-
Superior column performance and longevity with OBD technology
-
Particle sizes: 5 µm and 10 µm
-
Produced under strict synthesis and column packing control
-
Designed for over 1000 DMSO injections
-
Mobile phases of low pH
-
Longer column lifetime with OBD technology
-
Increased loadability
-
Optimal retention of polar compounds
-
Compatible with 100% aqueous mobile phases
-
Particle sizes: 5 µm and 10 µm
-
Excellent chemical stability for both low- and high-pH purifications
-
Direct transition from lead generation to lead optimization in drug development
-
Excellent peak shapes for basic compounds
-
Increased mass loading, up to 60x compared to conventional silica-based materials
-
Hybrid particle technology
Increase preparative column lifetime with OBD technology
The lifetime of your preparative HPLC columns shouldn’t be random. With the extensive development of column hardware and packing process for OBD preparative columns, Waters has achieved predictable uniform density throughout the column and maximized mechanical stability. These extended column lifetimes and high mass loading capacity lead to increased productivity for our customers, who have come to rely on the high purity and recovery they can obtain with these columns. Scientists select OBD preparative columns for robustness, predictable performance, and ease of scaling from analytical to prep.
Prevent leaks with OBD preparative column design
Prevent leaks at high operating pressures with OBD preparative columns, which are designed to incorporate a pair of specially designed distributors and chemically-inert seals.
Directly scale UPLC methods to preparative HPLC columns
Successful scaling of methods from analytical to preparative dimensions requires the use of chromatographically equivalent columns. All too often, even when the same chemistry phase and particle size are used, methods do not scale either due to loss of resolution and/or lower than expected loading. By matching the analytical and preparative column bed densities, scalability is assured. OBD preparative columns are packed to bed densities that closely match the equivalent analytical column, providing excellent stability, reproducibility, and efficiency.
Discover the mass loading capacity for preparative OBD columns
Mass capacity of preparative columns can be affected by many factors. In general, mass loading capacity is typically higher for strongly retained material, higher for simple mixtures, lower where higher resolution is required, and they are very strongly dependent on loading conditions. For more detailed instructions on scaling-up, visit our Preparative OBD Column Calculator.
Simplify peptide isolation and purification
Unpredictable separations, short column lifetimes, and lack of scalability can make peptide isolation and purification difficult. Peptide columns provide a stable surface chemistry that can be used for most peptides. Scaling the optimized separation requires identical column chemistry and performance across a range of column dimensions. With OBD preparative column design and the stable surface chemistry of Waters peptide columns, in a range of particle sizes, you can improve the efficiency of peptide isolation and purification processes and reduce overall costs. For more information, read our Practical Approaches to Peptide Isolation.
Highly efficient and reproducible oligonucleotide purification with improved yields and turnaround time
Waters batch-certified Bridged Ethyl Hybrid (BEH) technology for oligonucleotides ensures extra performance, reproducibility, and scale-up for purification. High temperature and high pH are prevalent strategies for minimizing secondary interactions in oligonucleotide separation and purification. BEH particle technology withstands harsh conditions such as high pH, temp and additives that usually are required for oligonucleotide work.
Batch-tested and QC-certified XBridge Oligonucleotide OBD Prep Columns provide extra assurance for reproducibility, method transfer and support emerging purification needs for standard and new chemically diverse siRNAs, ASOs, and oligonucleotide reagents. Each batch of oligonucleotide BEH C18 sorbent is QC-tested and selected using the Waters MassPrep Oligonucleotide Standard and a stringent resolution and peak tailing assessment from a triethylammonium acetate IP-RP separation. This carefully examined sorbents are available in both 2.5 and 5 µm particle sizes as well as 130 and 300 Å pore sizes to give oligonucleotide chemists the range of versatile options they need to purify a range of different synthesis products. Purifying Oligonucleotide Solution Guide
Extend column lifetimes with preparative guards
The use of preparative guards will greatly extend preparative column lifetimes. Guard columns are easy to install ahead of the preparative column and provide superior protection. Waters preparative guard holders and cartridge assemblies are available in 10 mm length in four different inner diameters including 7.8, 10, 19, and 30 mm. These guard holders and cartridge assemblies protect Waters OBD Preparative Columns with the same internal diameter, as shown with these options:
- 7.8 x 10 mm cartridges and 7.8 x 10 mm Cartridge Holder
- 10 x 10 mm cartridges and 10 x 10 mm Cartridge Holder
- 19 x 10 mm cartridges and 19 x 10 mm Cartridge Holder
- 30 x 10 mm cartridges and 30 x 10 mm Cartridge Holder
Resources
Documents
Support
Support
What do you want to do?
Related
Offers and Discounts
Preparative HPLC columns and components are specialized elements designed for the purification and isolation of compounds on a larger scale compared to analytical HPLC. These integral parts play a pivotal role in ensuring effective separations and obtaining high-purity fractions in preparative chromatography. Below is an in-depth overview:
Preparative HPLC Columns:
-Variety of Column Types: Available in reversed-phase, normal phase, ion exchange, size exclusion, and affinity types, the selection is influenced by the sample's nature and the desired separation mechanism.
-Column Dimensions: Distinguished by larger dimensions, including increased length and diameter, preparative HPLC columns enable higher sample loads and enhanced resolution compared to analytical counterparts.
Column Packing Material:
-Critical Stationary Phase: The stationary phase's role in preparative columns is pivotal for successful separations. It may be composed of silica-based, polymer-based, or other materials tailored for specific applications.
-Optimized Particle Size: In contrast to smaller particles offering higher resolution in analytical columns, preparative columns often use larger particles to handle increased sample loads without incurring excessive backpressure.
Preparative HPLC Parts:
-Protective Guard Columns: These safeguard the primary preparative column against contamination, prolonging its lifespan by capturing impurities before reaching the analytical column.
-Sturdy Column Hardware: Encompassing the column housing, fittings, and connectors, these components are engineered to withstand the elevated pressures associated with preparative chromatography.
-Frits and Filters: Essential components aiding in retaining the stationary phase within the column and preventing the ingress of particles into the HPLC system.
Preparative HPLC Systems:
-Automated Sample Loading: Commonly integrating automated sample loading features, prep HPLC systems ensure precision and reproducibility in injecting larger sample volumes onto the column.
-Efficient Fraction Collectors: Automated systems streamline the isolation process by effectively collecting purified fractions.
Column Care and Maintenance:
-Routine Cleaning Procedures: Essential for maintaining optimal performance and extending column life, regular cleaning of preparative columns is dictated by the sample's nature and the type of stationary phase.
-Backpressure Monitoring: Crucial in preparative chromatography, monitoring and controlling backpressure prevent column damage and ensure efficient separations.
Application Considerations:
-Sample Compatibility: Examining properties like solubility, chemical stability, and compatibility with the chosen stationary phase is critical.
-Optimization Strategies: Parameters such as flow rate, mobile phase composition, and gradient profile are tailored for specific applications.
The careful selection and maintenance of preparative HPLC columns and parts significantly contribute to the successful purification of larger quantities of compounds across diverse applications, including pharmaceutical development, natural products research, and industrial processes.
Preparative HPLC Columns & Parts FAQs
1.How do I choose the right Preparative HPLC Column for my application?
Choosing the right Preparative HPLC Column is an important consideration that is based on several factors. First, identify the type of sample you're working with, such as proteins, peptides, or small molecules. The column's particle size and pore size are crucial for achieving the desired resolution and load capacity. For high-resolution separations, smaller particle sizes are ideal, while larger particles may be better for higher load capacities.
Also, consider the scale of your purification—larger columns are needed for higher volumes. Waters offers a range of columns optimized for different applications, so matching your specific needs with the appropriate column is essential for optimal results.
2.How do I maintain the longevity of my Preparative HPLC Column?
Proper handling and regular maintenance are non-negotiable to maintain the longevity of your Preparative HPLC Column. This starts with always using high-quality solvents and buffers to avoid contamination and buildup. After each use, flush the column with a suitable solvent to remove residual samples and prevent blockages.
Store the column in an appropriate solvent to maintain the stability of the packing material and avoid exposure to extreme temperatures and pressures that can damage the column. Regular monitoring of column performance, such as checking for pressure increases or changes in resolution, can help identify issues early and prolong the column's life.
3.What is the importance of particle size and pore size in Preparative HPLC Columns?
Smaller particle sizes generally provide higher resolution, allowing for better separation of closely related compounds. However, they may also lead to higher back pressure, which needs to be managed. Pore size determines the size of molecules that can be effectively separated. For larger biomolecules like proteins, larger pores are necessary to prevent exclusion and ensure efficient separation. Understanding the balance between particle and pore size is essential for optimizing column performance, achieving high purity, and maintaining the efficiency of the preparative process.
