• Application Note

Codeine and its Glucuronide Metabolite in Plasma

Codeine and its Glucuronide Metabolite in Plasma
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights on the analysis of codeine and its glucuronide metabolite in plasma.

Introduction

Experimental

HPLC Conditions

Column:

Symmetry C18, 2.1 x 150 mm, 3.5 μm

Guard column:

Symmetry C18, 2.1 x 10 mm, 3.5 μm

Part numbers:

Column - 186000174, Guard - 186000169

Mobile phase:

Methanol/Acetonitrile/0.05% TFA in water 5:5:90

Flow rate:

0.3 mL/min

Injection volume:

80 μL Plasma extract

Temperature:

30° C

Detection:

UV @ 220 nm (0.04 AUFS)

Results and Discussion

The compounds analyzed in this study are:

  1. Ranitidine (I.S.)
  2. Codeine-6-Glucuronide (C-6-G) 
  3. Codeine

Chromatogram of Plasma Extracts: A) Blank B) Spiked Sample

Featured Products

WA31764.64, June 2003

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