• Application Note

Phenone Mixture

Phenone Mixture
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of phenone mixture using Sunfire C8 and C18 Columns.

Introduction

The compounds analysed in this study using Sunfire C18 Columns are -

II compound mixture of phenones separated in under 4 minutes with SunFire C18 IS Column.

The compounds analysed in this study using Sunfire C8 Columns are -

*: stock solution prepared in acetontrile
II compound mixture of phenones separated in under 4 minutes with SunFire C8 IS Column.

Experimental

Conditions

Column:

SunFire C18 4.6 x 20 mm IS, 3.5 μm

Part number:

186002549

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in ACN

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Conditions

Column:

SunFire C8 4.6 x 20 mm IS, 3.5 μm

Part number:

186002699

Mobile phase A:

Water

Mobile phase B:

Acetonitrile

Mobile phase C:

1% HCOOH in Water

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Sample concentration:

10 μg/mL in water

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Results and Discussion

Compound mixture of phenones separated in under 4 minutes with SunFire IS Column.

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WA41897, August 2005

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