• Application Note

Analysis of Estradiol USP Method Using XBridge C18

Analysis of Estradiol USP Method Using XBridge C18


  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of estradiol USP method using XBridge C18 Columns. 

Introduction

Compounds

1. Ethylparaben 
2. Estradiol 
3. Estrone 

Experimental

Test Conditions

Column:

XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116

Isocratic Mobile Phase:

water/acetonitrile (45:55)

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Sample Concentration:

20 μg/mL in water/ACN (50:50)

Temperature:

25 ˚C

Detection:

UV @ 205 nm

Instrument:

Alliance 2695 with 2996 PDA

Results and Discussion

1. Ethylparaben 2. Estradiol 3. Estrone.

Featured Products

WA60164, April 2007

Back To Top Back To Top