This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of betamethasone.
The compounds used in this study are –
Compounds |
USP Tailing Factors |
---|---|
Betamethasone |
1.12 |
Propylparaben (I.S.) |
1.09 |
Column: |
Symmetry C8, 3.9 x 150 mm, 5 μm |
Guard column: |
Sentry Guard Column 3.9 x 20 mm, 5 μm |
Part numbers: |
Column - WAT046970, Guard - WAT054250 |
Mobile phase: |
Water/acetonitrile/phosphoric acid 63:37:0.1 |
Flow rate: |
1.0 mL/min |
Injection volume: |
10 μL of 50 μg/mL sample |
Detection: |
UV @ 254 nm |
WA31763.37, June 2003