Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 300 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Chemistry |
Diol (OH) |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
5000 psi (345 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Min |
100000 |
Molecular Weight Range Max |
1500000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
450 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
80 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
300 mm |
Carbon Load |
9 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |