This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of barbiturates in human urine.
The compounds used in this study are –
|
Column: |
Symmetry Shield RP18, 2.1 x 150 mm, 5 μm |
|
Guard column: |
Sentry Guard Column RP18, 3.9 x 20 mm, 5 μm |
|
Part number: |
Column - 186000111, Guard - 186000107 |
|
Mobile phase: |
50 mM potassium phosphate, pH 7.0/acetonitrile 71:29 |
|
Flow rate: |
1.0 mL/min |
|
Injection volume: |
80 μL urine extract |
|
Temperature: |
30 °C |
|
Detection: |
UV @ 214 nm (0.350 AUFS) |
WA31763.34, June 2003
