ACQUITY UPLC Analysis of Organic Acids

Column:

ACQUITY BEH Amide, 2.1 x 100 mm, 1.7 μm

Part Number:

186004801

Mobile phase A:

50/50 MeCN/H₂O with 10 mM CH₃COONH₄, pH 9.0

Mobile phase B:

95/5 MeCN/H₂O with 10 mM CH₃COONH₄, pH 9.0

Gradient Flow Rate:

0.6 mL/min

Injection Volume:

5.0 μL

Column Temp:

50 °C

Sample Temp:

5 °C

Strong/Weak needle wash:

95/5 MeCN/H₂O

Seal wash:

10/90 MeOH/H₂O

Instrument:

ACQUITY UPLC and TQD

Instrument:

ACQUITY TQD

Ionization Mode:

ES^-

Capillary Voltage:

4.0 kV

Cone Voltage:

-25 V

Collision Energy:

10 eV

Extractor:

3 V

RF Lens:

0.1 V

Source Temp:

130 °C

Desolvation Temp:

350 °C

Desolvation Gas:

650 L/hr

Cone Gas:

0 L/hr

Collision Gas:

0.1 mL/min

MRM condition:

Pyruvic acid: 86.92 > 42.9

Lactic acid: 88.92 > 42.9

Succinic acid: 116.93 > 72.9

Maleic and Fumaric acid: 114.88 > 70.9