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Imipramine

Imipramine
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of imipramine using XTerra Columns.  

Introduction

The compound analyzed in this study is imipramine. 

Experimental

Conditions

Column:

XTerra RP18 4.6 x 150 mm, 5 µm

Part number:

186000492

Mobile phase:

pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 40:50:10

pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 22:68:10

pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 90:0:10

Flow rate:

1.0 mL/min

Injection volume:

5 µL of 250 µg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile Phase pH

USP Tailing

3.0

1.24

7.0

1.49

10.0

1.19

Results and Discussion

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WA20738.063, June 2002

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