This is an Application Brief and does not contain a detailed Experimental section.
This application brief details on the analysis of barbiturates using Symmetry Columns.
The compounds analyzed in this study are:
Column: |
Symmetry C8, 3.9 x 150 mm, 5 µm |
Guard column: |
Symmetry Guard Column 3.9 x 20 mm, 5 µm |
Part numbers: |
Column - WAT046970, Guard - WAT054250 |
Mobile phase: |
100 mM Potassium phosphate, pH 6.9/acetonitrile/water 20:30:50 |
Flow rate: |
1.0 mL/min |
Injection volume: |
10 µL of 25µg/mL sample |
Detection: |
UV @ 214 nm |
WA31763.33, June 2003