This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates separation of a tryptic digest of phosphorylase B.
The compound used in this study is tryptic digest of phosphorylase B.
Column: |
BioSuite PA Series (2.1 x 150 mm) |
Eluent A: |
0.02% TFA in water |
Eluent B: |
0.016% TFA in acetonitrile |
Gradient: |
0–56% B in 70 minutes |
Flow rate: |
0.16 mL/min |
Injection volume: |
15 μL |
Temperature: |
40 °C |
Detection: |
UV 220 nm |
WA31792, June 2003