This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the analysis of benzamide using XTerra Columns.
In this study the compound analyzed is benzamide.
Column: |
XTerra RP18 4.6 x 50 mm, 5 µm |
Part number: |
186000492 |
Mobile phase: |
pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 50: 50:40:10 pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 10:10:10 pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 80:10:10 |
Flow rate: |
1.0 mL/min |
Injection volume: |
5 µL of 250 µg/mL |
Temperature: |
30 °C |
Detection: |
UV @ 270 nm |
Instrument: |
Alliance 2695, 2996 PDA |
Mobile Phase pH |
USP Tailing |
---|---|
3.0 |
1.19 |
7.0 |
1.12 |
10.0 |
1.09 |
WA20738.017, June 2002