• Application Note

Vitamins- Fat-Soluble

Vitamins- Fat-Soluble
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of fat soluble vitamins.

Introduction

The compounds used in this study are – 

Compounds 

USP Tailing Factors

Trans-Retinol (Vitamin A)

1.33

Cholecalciferol (Vitamin D3)

1.2

Alpha-Tocopherol (Vitamin E)

1.16

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 μm

Guard Column:

Sentry Guard Column 3.9 x 20mm, 5 μm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

Methanol/acetonitrile/water 45:45:10

Flow rate:

1.0 mL/min

Injection volume:

10 μL of 25 μg/mL sample

Detection:

UV @ 280 nm

Results and Discussion

Featured Products

WA31763.179, June 2003

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