High Oligonucleotide Recovery From Liver Tissue
High Oligonucleotide Recovery From Liver Tissue
This is an Application Brief and does not contain a detailed Experimental section.
Abstract
This application brief provides guidance on the extraction of therapeutic oligonucleotides from tissue samples for quantitative analysis and demonstrates high oligonucleotide recovery from Liver Tissue.
Experimental
The following (Figure 1) summarizes the solvent-assisted tissue homogenization and oligonucleotide extraction workflow and protocol we use internally with our OligoWorks SPE Microplate Kit. Note we utilize a 1:10 ratio between the mg of tissue being homogenized and the total volumne (mL) of homogenization mix, including Rapizyme Proteinase K digestion reagents, buffer and solvent.
Note: equipment referenced is what we use in our lab, but alternate equipment with equivalent capabilities may be used instead.
LC-MS Analysis
Results and Discussion
Ordering Information
Featured Products
Available for Purchase Online
- SKU: 186010614OligoWorks SPE Microplate KitOnline ordering is limited to specific Distributors. Please sign in or contact your sales representative.
- SKU: 186009484ACQUITY Premier Oligonucleotide C18 Column, 130Å, 1.7 µm, 2.1 x 50 mm, 1/pkOnline ordering is limited to specific Distributors. Please sign in or contact your sales representative.
- SKU: 186009184QuanRecovery with MaxPeak, 700 µL Plates, 25/pkOnline ordering is limited to specific Distributors. Please sign in or contact your sales representative.
- SKU: 186009452ACQUITY Premier BEH C18 Column, 1.7 µm, 2.1 x 50 mm, 1/pkOnline ordering is limited to specific Distributors. Please sign in or contact your sales representative.
720008270, April 2024