PeptideWorks Tryptic Protein Digestion Kits uniquely deliver automatable, high efficiency, reproducible peptide maps in under 2.5 hours, 4x faster than average home-brew methods. With one kit, protein digestion can be performed manually or automated on Andrew+ Pipetting Robot and is amenable to other automated platforms. In addition, this kit contains a novel chemically optimized recombinant trypsin, RapiZyme Trypsin, Mass Spectrometry (MS) Grade for quick, clean and complete digestions in 30 minutes without contaminating autolysis peaks which can further complicate data processing. This starter kit includes everything needed for protein denaturation, reduction, alkylation, desalting and digestion to run a minimum of 4 samples up to 96 samples if needed. The kit provides: (2) bottles of Guanidine Hydrochloride (GuHCl) 5.7 g for denaturation, (8) bottles of Dithiothreitol (DTT) 16 mg for reducing, (8) bottles of Iodoacetamide (IAM) 53mg for alkylating, (12) x 8/pks of Sep-Pak SEC Desalting Cartridges with (1) 1 cc Cartridge Stand, (2) vials of 1M CaCl2 Salt Concentrate for desalting/digestion buffer and (4) vials RapiZyme Trypsin.
Customer will receive two packages. One package will ship cold-chain with all reagents and RapiZyme Trypsin. The second will ship ambient room temperature with the Sep-Pak SEC Desalting Cartridges and components.
