High Oligonucleotide Recovery From Liver Tissue
High Oligonucleotide Recovery From Liver Tissue
This is an Application Brief and does not contain a detailed Experimental section.
Abstract
This application brief provides guidance on the extraction of therapeutic oligonucleotides from tissue samples for quantitative analysis and demonstrates high oligonucleotide recovery from Liver Tissue.
Experimental
The following (Figure 1) summarizes the solvent-assisted tissue homogenization and oligonucleotide extraction workflow and protocol we use internally with our OligoWorks SPE Microplate Kit. Note we utilize a 1:10 ratio between the mg of tissue being homogenized and the total volumne (mL) of homogenization mix, including Rapizyme Proteinase K digestion reagents, buffer and solvent.
Note: equipment referenced is what we use in our lab, but alternate equipment with equivalent capabilities may be used instead.
LC-MS Analysis
Results and Discussion
Ordering Information
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720008270, April 2024