ACQUITY UPLC Systems with 2D LC Technology

Number of solvents

Up to four, in any combination of two: A1 or A2 and B1 or B2

Solvent storage

Solvent tray accommodates up to four chromatographic solvents, one Sample Manager wash solvent, and one Binary Solvent Manager seal wash solvent

Solvent conditioning

Integrated vacuum degassing, six lines with two allocated for the injector needle wash/purge solvents

Gradient formation

High pressure mixing, binary gradient

Gradient profiles

11 gradient curves [including linear, step (2), concave (4), and convex (4)]

Operating flow rate range

0.001 to 2.000 mL/min, in 0.001 mL increments (firmware version 1.71 and later)

Maximum operating pressure

18,000 psi up to 1 mL/min, 12,000 psi up to 2 mL/min

Primary check valves

Intelligent Intake Valves (i² Valve)

Flow accuracy†

±1.0% of set flow rate at 0.500 mL/min, as per SystemsQT

Flow precision†

≤0.075% RSD or 0.01 min SD, (0.2 to 2.0 mL/min), whichever is greater using pre-mixed solvent

Composition ripple† (baseline noise)

≤1.0 mAu

Compositional precision†

≤0.15% RSD, or 0.01 min SD, whichever is greater

Compositional accuracy†

±0.5% absolute from 5% to 95%, 0.2 to 2.0 mL/min

Pressure pulsation

≤0.4% or 25 psi, whichever is greater

Compressibility compensation

Automatic, no user intervention required

Priming

Wet priming runs at a flow rate of 4 mL/min

Effective system delay volume

<140 μL, independent of system back pressure (with 50 μL mixer installed)

Pump seal wash

Equipped with a programmable active wash system to flush the rear of the high-pressure seals and the plungers

Flow ramping

Automatic

Primary wetted materials

316 L stainless-steel, UHMWPE blend, MP35N, titanium alloy, gold, sapphire, ruby, zirconia, Nitronic 60, DLC, fluoropolymer, PEEK, and PEEK blend

Mixing options

Standard: 50 μL

Optional: 100 μL and 380 μL

Number of solvents

One to four, in any combination as standard.

Expanded solvent choices with optional six-port solvent select valve

Maximum operating pressure

15,000 psi up to 1.0 mL/min, 9000 psi up to 2.0 mL/min (firmware version 1.5x and earlier)

15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min (firmware version 1.6x and later)

Solvent conditioning

Integrated vacuum degassing, four chambers.

One additional for the SM-FTN-I purge solvent

Solvent blending

Automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto•Blend Plus Technology

Gradient formation

Low-pressure mixing, quaternary gradient

Gradient profiles

11 gradient curves (including linear, step [2], concave [4], and convex [4])

Settable flow rate range

0.010 to 2.000 mL/min, in 0.001 mL increments (firmware version 1.5x and earlier)

0.010 to 2.200 mL/min, in 0.001 mL increments (firmware version 1.60)

0.001 to 2.200 mL/min in 0.001 mL increments (firmware version 1.65 and later)

Primary check valve

Intelligent Intake Valve (i² Valve)

Pressure pulsation†

≤1.0% or 25 psi, whichever is greater

Flow accuracy†

±1.0% at 0.5 to 2.0 mL/min using 100% A

Flow precision†

≤0.075% RSD or ±0.01 min SD, whichever is greater, based on six replicates (with i² Valve)

Composition ripple† (baseline noise)

≤1.0 mAu

Composition accuracy†

±0.5% absolute (full scale) from 5% to 90% from 0.5 to 2.0 mL/min (with i² Valve)

Composition precision†

≤0.15% RSD or ±0.02 min SD, whichever is greater, based on six replicate injections (with i² Valve)

Compressibility compensation

Automatic and continuous

Priming

Wet priming can run at flow rates up to 4 mL/min. Programable from UPLC console software

Pump seal wash

Equipped with a wash system to flush the rear of the high pressure seal and the plunger, integration, and programable

Flow ramping

Range: 0.01 to 30.00 min to reach 2.0 mL/min

Default: 0.45 min to reach 2.0 mL/min

Primary wetted materials

316 L stainless-steel, PPS, fluoropolymer, fluoroelastomer, UHMWPE blend, sapphire, ruby, zirconia, Nitronic 60, DLC, PEEK, and PEEK blend, titanium alloy

Injection volume range

0.1 to 10.0 μL as standard configuration.

Up to 1000.0 μL with optional extension loop

Accuracy

±0.2 μL, measured by fluid weight removed from vial with 10.0 μL injections averaged over 20 injections using standard 100.0 μL syringe

Linearity†

≥0.999

Precision†

≤0.25%, 5 to 50 μL

Maximum sample capacity

Any two of the following:

• 96 and 384 microtiter plates

• 48 position 2.00 mL vial plates

• 48 position 0.65 mL micro-centrifuge tube plates

• 24 position 1.50 mL micro-centrifuge tube plates

Sample compartment temperature range

4.0 to 40.0 °C, settable in 0.1 °C increments; maintains 19 °C below ambient with a temperature range tolerance range between -2 and +4 °C

Temperature accuracy

±0.5 °C at sensor

Temperature stability

±1.0 °C at sensor

Sample manager heat time

≤30 min ambient-40 °C

Sample manager cool time

≤60 min ambient-4 °C

Injection needle wash

Integrated, active, programmable

Minimum sample required

3 μL residual, using Waters’ Total Recovery 2 mL Vials (zero offset)

Sample carryover

≤0.001% caffeine (UV)

≤0.001% sulphadimethoxine (MS)

Advanced sample manager capabilities

Auto-dilution and auto-addition

Primary wetted materials

316 L stainless-steel, polyimide, PEEK blend, DLC, PPS