Large Molecule Bioanalysis

Large Molecule Bioanalysis

Address the challenges of transitioning to peptide, protein, and oligonucleotide bioanalysis with Waters bespoke chemistries, optimized chromatography, and flexible high-performance mass spectrometry technologies. Waters UPLC-MS/MS large molecule solutions combine sensitivity, reproducibility, and productivity for optimized quantitative performance across the DMPK modality landscape.

Address the challenges of transitioning to peptide, protein, and oligonucleotide bioanalysis with Waters bespoke chemistries, optimized chromatography, and flexible high-performance mass spectrometry technologies. Waters UPLC-MS/MS large molecule solutions combine sensitivity, reproducibility, and productivity for optimized quantitative performance across the DMPK modality landscape.

Learn how to achieve high performance oligonucleotide preparative chromatography by utilizing XBridge Oligonucleotide OBD Prep Columns and efficient method optimization considerations
Learn how to achieve high performance oligonucleotide preparative chromatography by utilizing XBridge Oligonucleotide OBD Prep Columns and efficient method optimization considerations

Overview

Bioanalysis of biopharmaceuticals like peptides, proteins, and oligonucleotides present DMPK scientists with new challenges in sample isolation, separation, detection, and throughput. The complex nature of biopharmaceuticals requires scientists to solve the problems of analyte chromatographic absorption, sample processing, mass spectrometry (MS) method development, and data analysis.

Waters advanced chromatography, sample preparation kits, standards, software, and high sensitivity mass spectrometry solutions are designed to simplify the process of method development and ensure confident routine analysis throughout the drug development pipeline.


Support the expansion of biological therapeutics with Waters bespoke large molecule quantitative workflows that integrate seamlessly into your bioanalysis laboratory.

Applications

Oligonucleotides represent an ever-growing proportion of the pharmaceutical pipeline. Learn how Waters comprehensive suite of analytical solutions supports bioanalysis across the analytes of biological therapies in these application notes.

Oligonucleotides represent an ever-growing proportion of the pharmaceutical pipeline. Learn how Waters comprehensive suite of analytical solutions supports bioanalysis across the analytes of biological therapies in these application notes.


Application Notes

Application Notes


eBook: Sample Extraction and LC Tools for Reliable LC-MS Quantitation of Oligonucleotide

Oligonucleotide therapeutics operate at the level of gene transcription and translation and are providing opportunities to address inherited disorders and difficult to treat ailments such as cardiovascular disease. Learn how Waters OligoWorks solid phase extraction (SPE) kits and components help perfect sample preparation for LC-MS-based quantitation of oligonucleotides used in therapeutic delivery.

Oligonucleotide therapeutics operate at the level of gene transcription and translation and are providing opportunities to address inherited disorders and difficult to treat ailments such as cardiovascular disease. Learn how Waters OligoWorks solid phase extraction (SPE) kits and components help perfect sample preparation for LC-MS-based quantitation of oligonucleotides used in therapeutic delivery.


Oligonucleotide illustration

Solutions


Waters UPLC-MS/MS systems deliver absolute performance in separation and sensitivity for bioanalysis of proteins, peptides, and oligonucleotides.

Waters UPLC-MS/MS systems deliver absolute performance in separation and sensitivity for bioanalysis of proteins, peptides, and oligonucleotides.

Confident detection and quantification of large molecule analytes in biological matrices

Confident detection and quantification of large molecule analytes in biological matrices

Expand your analytical capabilities with the Xevo TQ-Absolute. Bringing superior performance and absolute sensitivity for robust quantification of large molecules with our highest-performing compact and sustainable tandem quadrupole mass spectrometer.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Dependable high performance chromatography for large molecules

Dependable high performance chromatography for large molecules

Optimize chromatographic resolution and speed while eliminating concerns of non-specific binding for peptide, protein, and oligonucleotide analytes with Waters ACQUITY Premier UPLC System, enhancing quantitative performance in bioanalysis assays.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Accessible optimized sensitivity for quantitation of large molecules

Accessible optimized sensitivity for quantitation of large molecules

Enhance sensitivity for low-volume peptide and protein samples with Waters ionKey Source with ACQUITY M-Class System, delivering the robustness and ease of implementation required for quantitative bioanalytical applications.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Let your LC inlet do the work for your nano- to micro-scale separations

Let your LC inlet do the work for your nano- to micro-scale separations

Achieve the performance you expect from nano- to microscale separations with the usability of an analytical-scale UPLC system with ACQUITY UPLC M-Class System.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis


The data speaks for itself

The data speaks for itself
Demonstration of improved out-of-the-box chromatographic performance (oligonucleotide recovery) for the GEM91 using the ACQUITY Premier Oligonucleotide C18 vs standard ACQUITY UPLC Oligonucleotide BEH C18 Column Demonstration of improved out-of-the-box chromatographic performance (oligonucleotide recovery) for the GEM91 using the ACQUITY Premier Oligonucleotide C18 vs standard ACQUITY UPLC Oligonucleotide BEH C18 Column.
Representative chromatograms of lowest calibration standards of oligonucleotides analyzed in plasma by Acquity Premier / Xevo TQ Absolute. Representative chromatograms of lowest calibration standards of oligonucleotides analyzed in plasma by ACQUITY Premier/ Xevo TQ Absolute.
MRM optimization data acquired on the Xevo TQ-XS and imported into Skyline. MRM optimization data acquired on the Xevo TQ-XS and imported into Skyline.
Mitigation of hydrophobic NSB to collection vessels using QuanRecovery™ with MaxPeak HPS 96-well Sample Collection plate, demonstrating improved MS peak response vs standard polypropylene plates Mitigation of hydrophobic NSB to collection vessels using QuanRecovery™ with MaxPeak HPS 96-well Sample Collection plate, demonstrating improved MS peak response vs standard polypropylene plates.

Webinars and Resources



  • Journal Citations

Practical Applications of Integrated Microfluidics for Peptide Quantification

Practical Applications of Integrated Microfluidics for Peptide Quantification
  • Infographic

Large Molecule Analysis by LC-MS–Survey Infographic

Large Molecule Analysis by LC-MS–Survey Infographic
  • Application Notebook

Peptide and Protein Bioanalysis 

Peptide and Protein Bioanalysis 
  • Application Notebook

Versatile LC-MS/MS Solutions for the Evolving Bioanalysis Landscape

Versatile LC-MS/MS Solutions for the Evolving Bioanalysis Landscape
  • eBook

Quantifying Oligonucleotides eBook

Quantifying Oligonucleotides eBook

Related

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Accurately perform small molecule bioanalysis of drug therapeutics with Waters UPLC-MS/MS solutions, designed to maximize throughput and minimize non-specific absorption.

Learn more about Large Molecule Bioanalysis Solutions.

Learn more about Large Molecule Bioanalysis Solutions.

Learn how to achieve high performance oligonucleotide preparative chromatography by utilizing XBridge Oligonucleotide OBD Prep Columns and efficient method optimization considerations