Amino acids are basic building blocks of proteins. Analyzing amino acids is required in various areas of research and development (R&D) and is fundamental in product analysis for food, beverages, and biotherapeutics. However, analyzing amino acids poses unique challenges due to their chemical and physical properties.
Overcome the challenges of maximizing protein yield and maintaining manufacturing consistency with Waters, with amino acid solutions to ensure precision and high throughput, addressing the needs of protein characterization, cell culture monitoring, and nutritional analysis of foods and feeds. Designed with exceptional reliability, Waters end-to-end solutions enable you to achieve precise qualitative and quantitative measurement of amino acids with exceptional sensitivity. Your lab benefits from shorter analysis time, method robustness to minimize variability, and accurate, confident results.
Application Notebook: Amino Acid Analysis
Get the flexibility of quaternary or binary solvent blending with the advanced performance of UPLC technology with ACQUITY UPLC H-Class PLUS System, delivering increased resolution, sensitivity, and speed that comes with modern small particle chemistries.
Maximize the sensitivity, repeatability, and robustness of your LC and LC-MS applications with the ACQUITY Premier System that features MaxPeak High Performance Surfaces (HPS) Technology for improvements in the separation and detection of metal-sensitive analytes.
Produce the most accurate, reliable, and reproducible data with the Arc Premier System for right first time robust results that features MaxPeak High Performance Surfaces (HPS) Technology to reduce non-specific adsorption due to metal interaction.
Enable LC-MS biopharmaceutical analyses in every lab with Waters BioAccord LC-MS System, including high-resolution MS and waters_connect software for compliance-ready data analysis and reporting.
Analysis of Protein Hydrolysate standard on the AccQ•Tag Ultra C18, 2.5 µm 4.6 x 150 mm Column batch-to-batch reproducibility.
Analysis of a hydrolysate standard at 10 pmol/mL. The UPLC Amino Acid Analysis Solution provides high resolution to ensure method robustness with good quantitation. The short cycle time is obtained without compromising accurate results.
Representative chromatogram of the amino acid cell culture standard acquired on the ACQUITY UPLC H-Class PLUS Binary System. The sample concentration was 500 µM for all amino acids, except cysteine which was 250 µM.
Protein Hydrolysate AA Standard (500 µM) using an AccQ•Tag Ultra C18 2.5 µm Column on an ACQUITY Arc System.
Protein Hydrolysate AA Standard (500 µM) chromatogram using an AccQ•Tag Ultra C18 2.5 µm Column on an Arc HPLC System.
Schematic overview of sample preparation, LC-MS analysis, and report for cell culture media nutrient and metabolite analysis based on Andrew+ Pipette Robot and BioAccord LC-MS platform.
