Structural Biology

Structural Biology

Revolutionize your protein and protein complex analysis with ion mobility enabled time-of-flight (Tof) mass spectrometry (MS) solutions from Waters, which help your laboratory generate important breakthroughs in the determination of overall size, shape, and subunit architecture of protein complexes.

Revolutionize your protein and protein complex analysis with ion mobility enabled time-of-flight (Tof) mass spectrometry (MS) solutions from Waters, which help your laboratory generate important breakthroughs in the determination of overall size, shape, and subunit architecture of protein complexes.

Achieve unique molecular insights into structural biology and elucidate the gas phase structure of protein macromolecular complexes with Waters structural biology solutions. 
Achieve unique molecular insights into structural biology and elucidate the gas phase structure of protein macromolecular complexes with Waters structural biology solutions. 

Overview

Achieving insights into the structure of proteins and protein macromolecular complexes is critical in understanding the relationship with their biological function. 

Using either HDX-MS or Native MS analysis, the SELECT SERIES Cyclic IMS can help your lab learn about the physical size and shape of proteins, and is ideal for modeling protein assembly structures that are difficult and time-consuming to study with conventional structural biology approaches.

For studying protein unfolding and subunit configurations, the Cyclic IMS can be configured with an array of ion activation techniques, including electron capture dissociation (ECD), surface induced dissociation (SID), and collision-induced dissociation (CID). All of these techniques combine with scalable ion mobility and IMSn to deliver unique insights in structure.


Cyclic IMS ESI angled

Applications

The higher order structure of protein biotherapeutics is an important attribute to measure in order to assure the effectiveness and safety of new therapies. Learn how Waters advanced structural biology toolset can be applied in the biopharmaceutical industry.

The higher order structure of protein biotherapeutics is an important attribute to measure in order to assure the effectiveness and safety of new therapies. Learn how Waters advanced structural biology toolset can be applied in the biopharmaceutical industry.


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Webinar: Advanced Characterization of Biotherapeutics Using Cyclic Ion Mobility-Mass Spectrometry

Hydrogen–deuterium exchange mass spectrometry (HDX-MS) is a powerful technique to study protein structure. Listen as Dale Cooper-Shepard demonstrates the SELECT SERIES Cyclic IMS as a highly versatile tool for biopharma research and how Cyclic IMS significantly increases protein coverage for detailed HDX studies.

Hydrogen–deuterium exchange mass spectrometry (HDX-MS) is a powerful technique to study protein structure. Listen as Dale Cooper-Shepard demonstrates the SELECT SERIES Cyclic IMS as a highly versatile tool for biopharma research and how Cyclic IMS significantly increases protein coverage for detailed HDX studies.


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Solutions


Waters LC-MS and GC-MS systems offer absolute performance in separation and sensitivity for PFAS quantitation and identification.

Waters LC-MS and GC-MS systems offer absolute performance in separation and sensitivity for PFAS quantitation and identification.

Endless possibilities, limitless structural biology research

Endless possibilities, limitless structural biology research

Interrogate heterogeneous protein assemblies in the 10 kDa to 1 MDa range with the SELECT SERIES Cyclic IMS ion mobility mass spectrometry, ideal for intact in the gas phase and for structural biology analysis.

  • Biotherapeutic Protein Characterization
  • Intact Proteins and Native MS

Ultimate flexibility for deeper discovery

Ultimate flexibility for deeper discovery

Improve your post-translational modification studies with the SYNAPT XS that uniquely implements electron-transfer dissociation (ETD) in the trap-region of the TriWave device for the optional, supplemental activation of ions by collision-induced dissociation (CID).

  • Biotherapeutic Protein Characterization
  • Intact Proteins and Native MS

Answers for all of your structural questions

Answers for all of your structural questions

Get the robust solution you need for protein drug discovery and development and higher order structure studies with Waters HDX MS LC-MS, automation, and informatics solutions.

  • Biotherapeutic Protein Characterization
  • Intact Proteins and Native MS

Redefine performance with all the benefits of time-of-flight technology

Redefine performance with all the benefits of time-of-flight technology

Accelerate your research and comprehensively identify analytes in complex samples with the SELECT SERIES MRT that provides a unique combination of ultra-high resolution and specificity independent of scan speed, for unambiguous compound identification with even the most complex samples.

  • Biotherapeutic Protein Characterization
  • Intact Proteins and Native MS


The data speaks for itself

The data speaks for itself
Extracted ion mobilogram at 597.34 m/z corresponding to the peptide GIIQPQQPAQL with one deamidated glutamine residue (out of a possible four). Based on drift time after five passes, four different species could be separated. Extracted ion mobilogram at 597.34 m/z corresponding to the peptide GIIQPQQPAQL with one deamidated glutamine residue (out of a possible four). Based on drift time after five passes, four different species could be separated.
Resolving deamidations for confident quantification. A) The deamidated forms of peptide HC:T23 elute in the tail of the native peptide peak.

Resolving deamidations for confident quantification. A) The deamidated forms of peptide HC:T23 elute in the tail of the native peptide peak.

Without the ultra-high resolution of the SELECT SERIES MRT as shown in (B) identification and quantification of the deamidations.

Representative MaxEnt1 deconvolution of trastuzumab from denaturing analysis (10 ng injection). Representative MaxEnt1 deconvolution of trastuzumab from denaturing analysis (10 ng injection).

Webinars and Resources



  • Brochure

Enhanced Biopharmaceutical Characterization Using the SELECT SERIES MRT– Product Solution

Enhanced Biopharmaceutical Characterization Using the SELECT SERIES MRT– Product Solution

Related

Answers for all of your structural questions for biotherapeutics, complexes, and protein folding/stabillity studies. Waters offers unrivaled instrumentation, chemistries, informatics, and training programs.

Waters proteomics solutions and expertise are leading resources for proteomics discovery research. Our MS-based systems maximize efficiency and provide unique tools for protein discovery, characterization, quantification, and validation.

Facilitate a smooth translation from multi-omics discovery to high-throughput, high-sensitivity quantitative LC-MS methods with Waters robust analytical solutions for targeted proteomics, lipidomics, and metabolomics applications.

Differentiate between samples and comprehensively identify proteins in your samples with Waters discovery – or nontargeted – proteomics solutions.

Learn more about Structural Biology Solutions.

Learn more about Structural Biology Solutions.

Achieve unique molecular insights into structural biology and elucidate the gas phase structure of protein macromolecular complexes with Waters structural biology solutions.